Background
The ColorMixed Protein Marker 180 is a ready to use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 170 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer).
This marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes, (PVDF nylon, or nitrocellulose) and for approximating the size of proteins.
Product Information
| Storage Temperature | 4°C for 3 months, -20°C for 2years |
|---|
Notice
- 1. Readyto use: Do NOT heat, dilute, or add reducing agents before Loading
- 2. Reference bands: 75 kDa (red), 25 kDa (green)
- 3. Loading Volume: 3-5 μL/well for mini gel; 1.5-2.5 μL/ well for Western transfer; 6-10 μL/ well for large gel
- 4. Type of membranes: PVDF nylon, or nitrocellulose
Guide for Molecular Weight
Migration patterns and approximate MWs (kDa) of ColorMixed Protein Marker 180 in different electrophoresis conditions are listed below:
| Band | Color | TRIS-GLYCINE / kDa | BIS-TRIS (MOPS) / kDa | BIS-TRIS (MES)/ kDa |  |
|---|
| 1 | Blue | 180 | 170 | 170 |
| 2 | Blue | 140 | 130 | 130 |
| 3 | Blue | 100 | 93 | 93 |
| 4 | Red | 75 | 70 | 72 |
| 5 | Blue | 60 | 53 | 53 |
| 6 | Blue | 45 | 41 | 42 |
| 7 | Blue | 35 | 30 | 30 |
| 8 | Green | 25 | 22 | 23 |
| 9 | Blue | 15 | 14 | 14 |
| 10 | Blue | 10 | 9 | 10 |
