HighGene transfection reagent (RM09014)

Figure 1: inoculated 293T, HCT116 and C6 cells in the 6-well cell plate with a cell density of about 80%. Used HighGene transfection reagent andtwo similar products on the market for cell transfection respectively. The amount of GFP eukaryotic expression plasmids was 2μg. Observed the cells under a fluorescence microscope and took photo for the cells 48 hours aftertransfection.

Figure 2: inoculated 293T and HeLa cells in the 6-well cell plate with a cell density of about 70%. The amount of GFP eukaryotic expression plasmids was 2μg and that of the two different batches of HighGene transfection reagent was 4μL in cell transfection. Observed the cells under a fluorescence microscope and took photo for the cells 24 hours after transfection.

Figure 3: inoculated 30mL of HEK293F with a density of 1 x10⁶ cells/mL or Sf9 suspension cell into a 125mL shake flask. The amount of GFP eukaryotic expression plasmids was 30μg and that of HighGene transfection reagent was 60μL in cell transfection. Observed the cells under a fluorescence microscope and took photo for the cells 48 hours after transfection.

Figure 4: inoculated HEK293T, HeLa and HCT116 cells in 96-well cell plate, with 5, 000 cells in each well and cell density of about 50%, and prepared 5 repeated wells. Treated cells with HighGene transfection reagent of 2μL/mL for 24h and 48h respectively and then used CCK8 reagent to test the cell viability.

Figure 5: inoculated 293T and HeLa cells in the 6-well cell plate with a cell density of about 80%. The amount of Cy3 siRNA was 100pmol and that of HighGene transfection reagent was 5μL, or that of YAP1 siRNA was 200pmol and that of HighGene transfection reagent was 10μL in cell transfection. Observed the cells under a fluorescence microscope and took photo (A) for the cells 24 hours after transfection or prepare cell sample to perform WB experiment (B). (Remark: YAP1 antibody used in WB experiment is from ABclonal, with a catalog No. A1002.)

Figure 6: inoculated 293T cells in the 6-well cell plate with a cell density of 80%. The amount of GFP eukaryotic expression plasmid was 2μg, that of Cherry eukaryotic expression plasmid was 2μg and that of HighGene transfection reagent was 8μL in cell transfection. Observed the cells under a fluorescence microscope and took photo for the cells 24 hours after transfection.

Figure 7: inoculated 293T cells in a 10cm culture dish with a cell density of 80%. Performed cell transfection for lentivirus packaging plasmids and external GFP expression plasmids, and got lentivirus liquid after 48 hours. Took 200uL lentivirus liquid to infect the A549 cells in the 6-well cell plate, observed the cells under a fluorescence microscope and took photo for the cells 48 hours after transfection.