2X MultiF Seamless Assembly Mix (RK21020)

A widespread single amino acid mutation in AcrA reduces tigecycline susceptibility in Klebsiella pneumoniae

Combining protein and metabolic engineering strategies for high-level production of L-theanine in Corynebacterium glutamicum

A transcription factor-based bacterial biosensor system and its application for on-site detection of explosives

Alternative Polyadenylation of Malic Enzyme 1 Is Essential for Accelerated Adipogenesis

An ULK1/2PXN mechanotransduction pathway suppresses breast cancer cell migration

LDLR is used as a cell entry receptor by multiple alphaviruses - PMC

RNA-binding protein RBM5 plays an essential role in acute myeloid leukemia by activating the oncogenic protein HOXA9 - PMC

High-level expression of leghemoglobin in Kluyveromyces marxianus by remodeling the heme metabolism pathway - PMC

EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses - PMC

Production of D-tagatose, bioethanol, and microbial protein from the dairy industry by-product whey powder using an integrated bioprocess

Significant Impact of AcrB Amino Acid Polymorphism at Residue 716 on Susceptibility to Tigecycline and Other Antibiotics in Klebsiella pneumoniae

Tracking pairwise genomic loci by the ParBParS and Noc-NBS systems in living cells

EPC1/2 regulate hematopoietic stem and progenitor cell proliferation by modulating H3 acetylation and DLST - PMC

Design of a dual-responding genetic circuit for high-throughput identification of L-threonine-overproducing Escherichia coli

Maize VKS1 Regulates Mitosis and Cytokinesis during Early Endosperm Development.

GPA peptide enhances Nur77 expression in intestinal epithelial cells to exert a 1 protective effect against DSS-induced colitis

African Swine Fever Virus A528R Inhibits TLR8 Mediated NF-B Activity by Targeting p65 Activation and Nuclear Translocation

Identification of imidazoquinoline derivative (IQD) interacting sites of porcine TLR8 and the underlying species specificity

The Signal Peptide and Chaperone UNC93B1 Both Influence TLR8 Ectodomain Intracellular Endosomal Localization

RNF4 controls the extent of replication fork reversal to preserve genome stability

Gly-pro-ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress

Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris

BPIFA2 as a Novel Early Biomarker to Identify Fatal Radiation Injury After Radiation Exposure

Mechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS

African Swine Fever Virus Structural Protein p17 Inhibits cGAS-STING Signaling Pathway Through Interacting With STING - PMC

VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion

VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion

Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering Pseudomonas Putida - PMC

The PRMT5/WDR77 complex restricts hepatitis E virus replication

Development of a Genetically Engineered Bivalent Vaccine against Porcine Epidemic Diarrhea Virus and Porcine Rotavirus - PMC

A Novel Mouse Model Harboring Hepatitis B Virus Covalently Closed Circular DNA

An Endogenous Retroviral LTR-Derived Long Noncoding RNA lnc-LTR5B Interacts With BiP to Modulate ALV-J Replication in Chicken Cells

The African swine fever virus protease pS273R inhibits DNA sensing cGAS-STING pathway by targeting IKK

The First Human Vulvar Intraepithelial Neoplasia Cell Line with Naturally Infected Episomal HPV18 Genome - PMC

Highly efficient biosynthesis of spermidine from L-homoserine and putrescine using an engineered Escherichia coli with NADPH self-sufficient system

Coordination of Polyketide Release and Multiple Detoxification Pathways for Tolerable Production of Fungal Mycotoxins

Enhanced production of aspochalasin D through genetic engineering of Aspergillus flavipes

An extracellular Cu/Zn superoxide dismutase from Neocaridina denticulata sinensis: cDNA cloning, mRNA expression and characterizations of recombinant protein

FfCOX17 is Involved in Fumonisins Production, Growth, Asexual Reproduction, and Fungicide Sensitivity in Fusarium fujikuroi - PMC

Construction of Coxsackievirus B5 viruses with luciferase reporters and their applications in vitro and in vivo

Construction and Application of a High-Throughput In Vivo Screening Platform for the Evolution of Nitrile Metabolism-Related Enzymes Based on a Desensitized Repressive Biosensor

miR319 and its target TCP4 involved in plant architecture regulation in Brassica napus

Insertion of a TRIM-like sequence in MdFLS2-1 promoter is associated with its allele-specific expression in response to Alternaria alternata in apple - PMC

Comparative biochemical characterization of mammalian-derived CYP11A1s with cholesterol side-chain cleavage activities

Programmable RNA base editing with a single gRNA-free enzyme - PMC

Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids - PMC

Selective degradation of cellular BRD3 and BRD4-L promoted by PROTAC molecules in six cancer cell lines

OGT Binding Peptide-Tagged Strategy Increases Protein O-GlcNAcylation Level in E. coli - PMC

Alleviating vacuolar transport improves cellulase production in Trichoderma reesei

A small molecule antagonist of SMN disrupts the interaction between SMN and RNAP II - PMC

MAVS-Based Reporter Systems for Real-Time Imaging of EV71 Infection and Antiviral Testing - PMC

A Cas12a ortholog with distinct TTNA PAM enables sensitive detection of HPV16/18 - PMC

The ATP Synthase Subunits FfATPh, FfATP5, and FfATPb Regulate the Development, Pathogenicity, and Fungicide Sensitivity of Fusarium fujikuroi - PMC

Transcription factor OsSNAC1 positively regulates nitrate transporter gene expression in rice

SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense

Reprogramming the Metabolic Network in Kluyveromyces lactis with a Transcriptional Switch for De Novo Lacto-N-biose Synthesis

Identification and Characterization of a Novel Quanzhou Mulberry Virus from Mulberry (Morus alba) - PMC

A fitness trade-off between growth and survival governed by Spo0A-mediated proteome allocation constraints in Bacillus subtilis - PMC

Role of cellulose response transporter-like protein CRT2 in cellulase induction in Trichoderma reesei - PMC

Purification and functional validation of LtCas12a protein - PMC

Rapid Visual Detection of African Swine Fever Virus with a CRISPR/Cas12a Lateral Flow Strip Based on Structural Protein Gene D117L - PMC

Porcine IKK is involved in the STING induced type I IFN antiviral response of the cytosolic DNA signaling pathway

High-efficiency genetic engineering toolkit for virus based on lambda red-mediated recombination

Alleviating symptoms of neurodegenerative disorders by astrocyte-specific overexpression of TMEM164 in mice

High-level production of D-arabitol by Zygosaccharomyces rouxii from glucose: Metabolic engineering and process optimization

NuRD mediates mitochondrial stressinduced longevity via chromatin remodeling in response to acetyl-CoA level

Upcycling of PET oligomers from chemical recycling processes to PHA by microbial co-cultivation

The transcription factor BnaA9.WRKY47 coordinates leaf senescence and nitrogen remobilization in Brassica napus

ProRefiner: an entropy-based refining strategy for inverse protein folding with global graph attention - PMC

MdAIL5 overexpression promotes apple adventitious shoot regeneration by regulating hormone signaling and activating the expression of shoot development-related genes - PMC

Flexible TAM requirement of TnpB enables efficient single-nucleotide editing with expanded targeting scope - PMC

Precision Engineering of the Transcription Factor Cre1 in Hypocrea jecorina ( Trichoderma reesei) for Efficient Cellulase Production in the Presence of Glucose

Mammalian IRE1 dynamically and functionally coalesces with stress granules

Modular assembly of an artificially concise biocatalytic cascade for the manufacture of phenethylisoquinoline alkaloids - PMC

Porcine TLR8 signaling and its anti-infection function are disturbed by immune checkpoint receptor TIM-3 via inhibition of P13K-AKT pathway

The Arabidopsis RTH plays an important role in regulation of iron (Fe) absorption and transport

A natural gene drive system confers reproductive isolation in rice

A non-canonical role of the inner kinetochore in regulating sister-chromatid cohesion at centromeres

The NTE domain of PTEN/ promotes cancer progression by interacting with WDR5 via its SSSRRSS motif - PMC

Identification of functional sgRNA mutants lacking canonical secondary structure using high-throughput FACS screening

Crucial Involvement of Heme Biosynthesis in Vegetative Growth, Development, Stress Response, and Fungicide Sensitivity of Fusarium graminearum - PMC

A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves - PMC

Targeting Nr2e3 to Modulate Tet2 Expression: Therapeutic Potential for Depression Treatment

The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm

The structural basis of the activation and inhibition of DSR2 NADase by phage proteins - PMC

FgPfn participates in vegetative growth, sexual reproduction, pathogenicity, and fungicides sensitivity via affecting both microtubules and actin in the

Engineering Chimeric Chemoreceptors and Two-Component Systems for Orthogonal and Leakless Biosensing of Extracellular -Aminobutyric Acid

The A226D Mutation of OmpC Leads to Increased Susceptibility to -Lactam Antibiotics in Escherichia coli - PMC

Specific Monoclonal Antibodies against African Swine Fever Virus Protease pS273R Revealed a Novel and Conserved Antigenic Epitope - PMC

A programmable CRISPR/dCas9-based epigenetic editing system enabling loci-targeted histone citrullination and precise transcription regulation

Cyclic di-GMP as an antitoxin regulates bacterial genome stability and antibiotic persistence in biofilms - PMC

DOT1L/H3K79me2 represses HIV-1 reactivation via recruiting DCAF1

The SUMO gene MrSmt3 is involved in SUMOylation, conidiation and stress response in Metarhizium robertsii - PMC

Genome-wide CRISPR/Cas9 library screen identifies C16orf62 as a host dependency factor for porcine deltacoronavirus infection - PMC

Molecular Basis of VCPIP1 and P97/VCP Interaction Reveals Its Functions in PostMitotic Golgi Reassembly - PMC

Zero-shot prediction of mutation effects on protein function with multimodal deep representation learning

Understanding energy fluctuation during the transition state: The role of AbrB in Bacillus licheniformis - PMC

Plasmid-encoded phosphatase RapP enhances cell growth in non-domesticated Bacillus subtilis strains - PMC

SRRM2 phase separation drives assembly of nuclear speckle subcompartments

Integrating genome wide association studies with selective sweep reveals genetic loci associated with tolerance to low phosphate availability in Brassica napus

Key amino acid residues govern the substrate selectivity of the transporter Xltr1p from Trichoderma reesei for glucose, mannose, and galactose

Identification of a hemorrhagic determinant in Clostridioides difficile TcdA and Paeniclostridium sordellii TcsH