Description
5'App-T4 DNA Ligase is a mutant of T4 DNA Ligase without adenylation function, which can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of DNA. This enzyme does not require ATP as a cofactor for ligation, but requires a pre-adenylated substrate. This enzyme reduces background ligation (chimera formation) during NGS library construction, because it can only use 5 'preadenylation adapter to the DNA fragments.
Product Features
- This enzyme does not require ATP as a cofactor，but requires a pre-adenylated substrate.
- It can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of DNA.
- More than 90% enzyme activity was maintained when the salt concentration reached 500 mM.
- strict batch performance and stability quality control.
Applications
This enzyme can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of DNA.
Ligation of 5 'preadenylation adaptor to DNA fragments for NGS library construction.
Storage
-20°C
Product Components
| Components | 20, 000 U | 100, 000 U |
| 5'App-T4 DNA Ligase (200, 000 U/mL) | 50 μL | 250 μL |
| 10X T4 DNA Ligase Reaction Buffer without ATP | 500 μL | 500 μL |
| 50% PEG8000 | 1.25 mL | 1.25 mL |
Documents
