제품 > 항체 > 단일클론항체(mAb)

ABflo® 450 Rabbit anti-Human CD80/B7-1 mAb (A26572)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293F cells (negative control, left) and Raji cells (right) were surface-stained with ABflo® 450 Rabbit anti-Human CD80/B7-1 mAb (A26572, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD80/B7-1 mAb (A26572, 5 μl/Test, right).

Overview

Product name	ABflo® 450 Rabbit anti-Human CD80/B7-1 mAb
Catalog No.	A26572
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61136-ABflo450

Background

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 35-242 of human CD80/B7-1 (NP_005182.1).
Sequence	VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN
Gene ID
Swiss Prot
Synonyms	B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Calculated MW	18kDa/29kDa/33kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell surface, external side of plasma membrane, plasma membrane.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

ABflo® 450 Rabbit anti-Human CD80/B7-1 mAb images

Flow CytoMetry - ABflo® 450 Rabbit anti-Human CD80/B7-1 mAb (A26572)

Flow cytometry: 1X10^6 Raji cells were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD80/B7-1 mAb (A26572, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.