제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Human CD80/B7-1 mAb (A23729)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293F cells (negative control, left) and Raji cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD80/B7-1 mAb(A23729, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 Raji cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD80/B7-1 mAb(A23729, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Human CD80/B7-1 mAb
Catalog No.	A23729
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61136-ABf488

Background

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 35-242 of human CD80/B7-1 (NP_005182.1).
Sequence	VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDN
Gene ID
Swiss Prot
Synonyms	CD80; B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7; CD80 molecule
Calculated MW	18kDa/29kDa/33kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell surface, external side of plasma membrane, plasma membrane

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 488 Rabbit anti-Human CD80/B7-1 mAb images

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD80/B7-1 mAb (A23729)

Flow cytometry:1X10^6 Raji cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD80/B7-1 mAb(A23729, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.