제품 > 항체 > 2차 항체

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS053)

Publications (100) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS053)

Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS053) at 1:200 dilution.
Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS053)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb (5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L)(AS053, 1:500）was used as a secondary antibody.

Overview

Product nameABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
Catalog No.AS053
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRabbit IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:100 - 1:500
  • FC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IHC(Crassostrea gigas, Rattus norvegicus, Other)

IF(Mus musculus, Rattus norvegicus, Homo sapiens, Ostreidae, Equus asinus, Oryza sativa, Sus scrofa, Capra hircus, Ctenopharyngodon idella, Danio rerio, Spodoptera frugiperda, Mus musculus, Arabidopsis thaliana, Rattus norvegicus, Escherichia coli, Oryctolagus cuniculus, Other)

WB(Homo sapiens, Other, Mus musculus, Triticum aestivum)

FC(Oryctolagus cuniculus, Mus musculus, Homo sapiens)

IF(Other)

Other(Sus scrofa, Bos taurus, Danio rerio, Mus musculus)

Enzyme assay(Danio rerio)

ELISA(Gallus gallus,Sus scrofa)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS053)}

    Immunofluorescence - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS053)

    Immunofluorescence analysis of HeLa cells, using ACO2 antibody (A3716) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
    Secondary antibody: ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS053) at 1:200 dilution.
    Blue: DAPI for nuclear staining.
    ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS053)}

    Flow CytoMetry - ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L) (AS053)

    Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb (5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L)(AS053, 1:500）was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS053? Please let us know so that we can cite the reference in this datasheet.