제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit IgG isotype control (A22070)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit IgG isotype control (A22070)

Flow cytometry:1X10^6 Human PBMC(negative control) were surface-stained with ABflo® 647 Rabbit anti-Human CD13/ANPEP mAb(A21945, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit IgG isotype control (A22070)

Flow cytometry:1X10^6 Human PBMC(negative control) were surface-stained with ABflo® 647 Rabbit anti-Human CD48 mAb(A21946, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC were used as blank control (red line).

Overview

Product nameABflo® 647 Rabbit IgG isotype control
Catalog No.A22070
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5105-10-ABf647
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.ABflo? 647 Excitation: 651 nm; Emission: 671 nm; Laser: Red Laser.
ImmunogenA synthesized peptide derived from rabbit IgG isotype control
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit IgG isotype control (A22070)}

    Flow CytoMetry - ABflo® 647 Rabbit IgG isotype control (A22070)

    Flow cytometry:1X10^6 Human PBMC(negative control) were surface-stained with ABflo® 647 Rabbit anti-Human CD13/ANPEP mAb(A21945, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit IgG isotype control (A22070)}

    Flow CytoMetry - ABflo® 647 Rabbit IgG isotype control (A22070)

    Flow cytometry:1X10^6 Human PBMC(negative control) were surface-stained with ABflo® 647 Rabbit anti-Human CD48 mAb(A21946, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC were used as blank control (red line).

    * For research use only. Not for therapeutic or diagnostic purposes.