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ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141)

Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141)

Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Myc-Tag mAb
Catalog No.AE141
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC71487-ABflo647
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Myc tag.
SequenceEQKLISEEDL
Gene ID
Swiss Prot
SynonymsMyc; Myc tag; Myc-tag
Calculated MW
Observed MW
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141)

    Flow cytometry: 1X10^6 293F cells (negative control, left) and 293F (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141)

    Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Myc-Tag mAb (AE141, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.