제품 > 항체 > 단일클론항체(mAb)

ABflo® 700 Rabbit anti-Mouse CD127/IL-7Rα mAb (A26546)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 700 Rabbit anti-Mouse CD127/IL-7Rα mAb (A26546, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Mouse CD127/IL-7Rα mAb (A26546, 5 μl/Test, right).

Overview

Product name	ABflo® 700 Rabbit anti-Mouse CD127/IL-7Rα mAb
Catalog No.	A26546
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63550-ABflo700

Background

Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 21-239 of mouse CD127/IL-7Rα(NP_612564.1).
Sequence	ESGNAQDGDLEDADADDHSFWCHSQLEVDGSQHLLTCAFNDSDINTANLEFQICGALLRVKCLTLNKLQDIYFIKTSEFLLIGSSNICVKLGQKNLTCKNMAINTIVKAEAPSDLKVVYRKEANDFLVTFNAPHLKKKYLKKVKHDVAYRPARGESNWTHVSLFHTRTTIPQRKLRPKAMYEIKVRSIPHNDYFKGFWSEWSPSSTFETPEPKNQGGWD
Gene ID
Swiss Prot
Synonyms	CD127; IL-7Ralpha
Calculated MW	51kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane.