제품 > 항체 > 다중클론항체(pAb)

ADNP Rabbit pAb (A4546)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ADNP Rabbit pAb (A4546)

Western blot analysis of extracts of various cell lines, using ADNP Antibody (A4546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - ADNP Rabbit pAb (A4546)

Immunofluorescence analysis of U-2 OS cells using ADNP Polyclonal Antibody (A4546) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameADNP Rabbit pAb
Catalog No.A4546
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Vasoactive intestinal peptide is a neuroprotective factor that has a stimulatory effect on the growth of some tumor cells and an inhibitory effect on others. This gene encodes a protein that is upregulated by vasoactive intestinal peptide and may be involved in its stimulatory effect on certain tumor cells. The encoded protein contains one homeobox and nine zinc finger domains, suggesting that it functions as a transcription factor. This gene is also upregulated in normal proliferative tissues. Finally, the encoded protein may increase the viability of certain cell types through modulation of p53 activity. Alternatively spliced transcript variants encoding the same protein have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 843-1102 of human ADNP (NP_056154.1).
SequenceEWLFENHDEKDSRVNASKTADKKLNLGKEDDSSSDSFENLEEESNESGSPFDPVFEVEPKISNDNPEEHVLKVIPEDASESEEKLDQKEDGSKYETIHLTEEPTKLMHNASDSEVDQDDVVEWKDGASPSESGPGSQQVSDFEDNTCEMKPGTWSDESSQSEDARSSKPAAKKKATMQGDREQLKWKNSSYGKVEGFWSKDQSQWKNASENDERLSNPQIEWQNSTIDSEDGEQFDNMTDGVAEPMHGSLAGVKLSSQQA
Gene ID
Swiss Prot
SynonymsADNP1; HVDAS; MRD28
Calculated MW124kDa
Observed MW163kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, U-87MG, HT-29, Mouse liver
Cellular locationNucleus

Documents

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ABclonal:Western blot - ADNP Rabbit pAb (A4546)}

Western blot - ADNP Rabbit pAb (A4546)

Western blot analysis of extracts of various cell lines, using ADNP Antibody (A4546) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - ADNP Rabbit pAb (A4546)}

Immunofluorescence - ADNP Rabbit pAb (A4546)

Immunofluorescence analysis of U-2 OS cells using ADNP Polyclonal Antibody (A4546) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)