제품 > 항체 > 다중클론항체(pAb)

ADRB3 Rabbit pAb (A8607)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using ADRB3 Rabbit pAb (A8607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - ADRB3 Rabbit pAb (A8607)

Immunofluorescence analysis of NIH/3T3 cells using ADRB3 Rabbit pAb (A8607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using ADRB3 Rabbit pAb (A8607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of C6 cells using ADRB3 Rabbit pAb (A8607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	ADRB3 Rabbit pAb
Catalog No.	A8607
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ADRB3 (NP_000016.1).
Sequence	MAPWPHENSSLAPWPDLPTLAPNTANTSGLPGVPWEAALAGALLALAVLATVGGNLLVIVAIAWTPRLQTMTNVFVTSLAAADLVMGLLVVPPAATLALT
Gene ID
Swiss Prot
Synonyms	BETA3AR; ADRB3
Calculated MW	44kDa
Observed MW	47kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HL-60, K-562
Cellular location	Cell membrane, Multi-pass membrane protein
Customer validation	WB(Mus musculus, Homo sapiens, Mus musculus) IHC(Homo sapiens) IF(Homo sapiens)

Documents

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Research Area

ADRB3 Rabbit pAb images

Western blot - ADRB3 Rabbit pAb (A8607)

Immunofluorescence - ADRB3 Rabbit pAb (A8607)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.279

Species:

Mus musculus

PMID:

31075194

Title:

A Combination of Capsaicin and Capsiate Induces Browning in 3T3-L1 White Adipocytes via Activation of the PPARγ/ β3-AR Signaling Pathways.

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

Nature communications

Application:

IF:

14.919

Species:

Mus musculus

PMID:

31147543

Title:

Adipose HuR protects against diet-induced obesity and insulin resistance.

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

Plant foods for human nutrition (Dordrecht, Netherlands)

Application:

IF:

4.12

Species:

Mus musculus

PMID:

35986845

Title:

Quercetin, Engelitin and Caffeic Acid of Smilax china L. Polyphenols, Stimulate 3T3-L1 Adipocytes to Brown-like Adipocytes Via β3-AR/AMPK Signaling Pathway

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

The American journal of Chinese medicine

Application:

IF:

Species:

Mus musculus

PMID:

35642460

Title:

Smilax china Polyphenols Stimulate Browning via [Formula: see text]3-Adrenergic Receptor/AMP-Activated Protein Kinase [Formula: see text] Signaling Pathway in 3T3-L1 Adipocytes

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

37468484

Title:

The muscle-enriched myokine Musclin impairs beige fat thermogenesis and systemic energy homeostasis via Tfr1/PKA signaling in male mice - PMC

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

Life sciences

Application:

IF:

6.78

Species:

Mus musculus

PMID:

37385371

Title:

Blocking ATP-P1Rs axis attenuate alcohol-related liver fibrosis

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

Analytical chemistry

Application:

IHC, IF

IF:

Species:

Homo sapiens

PMID:

36700797

Title:

Development and Characterization of a Highly Selective Turn-On Fluorescent Ligand for 3-Adrenergic Receptor

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus

PMID:

37804915

Title:

Miquelianin in Folium Nelumbinis extract promotes white-to-beige fat conversion via blocking AMPK/DRP1/mitophagy and modulating gut microbiota in HFD-fed mice

References for ADRB3 Rabbit pAb (A8607)

Product:

ADRB3 Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens, Mus musculus

PMID:

37680481