Description
This kit can be used for rapid extraction of RNA from various bacterial samples, and does not rely on toxic reagents such as phenol and chloroform. The unique lysis buffer rapidly lyses cells from trace samples and inactivates cell RNA enzymes. The lysate mixture passes through a gDNA Remove Column, where gDNA is removed and RNA is filtered through. After adjusting the binding conditions by ethanol, RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt. In a series of rapid washing-centrifugation steps, the purified RNA is eluted from the silicon membrane with low salt RNase-free H2O. The extracted RNA can be directly used in RT-PCR, qPCR and RNA library constructing experiments.
Product Features
- Safe: No need to use phenol, chloroform and other toxic reagents.
- Fast: Complete the extraction of several samples within 30 minutes.
- High purity: The obtained RNA has high purity and little genomic DNA residue, and can be directly used in downstream experiments with high purity requirements.
Applications
Extraction of RNA from various bacterial samples.
Storage
Reagents are stable for 12 months at room temperature.
Product Components
| Components | Catalog | Size
50 RXN |
| Buffer RL1 | RM30140 | 25 mL |
| Buffer PR2 | RM30141 | 35 mL |
| lysozyme | RM30205 | 20 mg |
| Buffer WB2 | RM30144 | 12 mL |
| RNase-free Adsorption Column and Collection Tubes | RM30185 | 50 pcs |
| gDNA Remove Column and Collection Tubes | RM30186 | 50 pcs |
| 1.5 mL RNase-free Centrifuge Tubes | RM30202 | 50 pcs |
| TE (pH 8.0) | RM30204 | 6 mL |
| RNase-free H2O | RM30142 | 5 mL |
Documents
