Description
Red blood cell lysis buffer selectively lyses red blood cells, and then the unique lysis buffer / ??-mercaptoethanol rapidly lyses white blood cells and inactivates cell RNA enzymes. After adjusting the binding conditions with ethanol, the RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt, and then the cell metabolites, proteins and other impurities are removed by a series of rapid washing-centrifugation steps. Finally the low-salt RNase-free H2O elutes the pure RNA from the silicon membrane.
Product Features
- Safe: No need to use phenol, chloroform and other toxic reagents.
- Fast: Complete the extraction of several samples within 40 minutes.
- Efficient: Repeated optimization of red blood cell lysis buffer, the lysis effect is fast and complete.
Applications
Rapid extraction of total RNA from whole blood.
Storage
Reagents are stable for 12 months at room temperature.
Product Components
| Components | Catalog | Size
50 RXN |
| Buffer RL3 | RM30210 | 30 mL |
| 10X Red Blood Cell Lysis Buffer | RM30203 | 25 mL |
| Buffer PR2 | RM30141 | 35 mL |
| RNase-free H2O | RM30142 | 5 mL |
| Buffer WB2 | RM30144 | 12 mL |
| RNase-free Adsorption Column and Collection Tubes | RM30185 | 50 pcs |
| 1.5 mL RNase-free centrifuge Tubes | RM30202 | 50 pcs |
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