This kit is capable of lysing cells using a modified SDS-alkaline lysis method. The crude extract is selectively bound with a unique endotoxin scavenger and centrifuged to remove the endotoxin. The silica-based membrane in the centrifugal adsorption column can bind the plasmid DNA in the solution selectively under the condition of high salt and low pH value, and then impurities and other bacterial components can be removed by the deproteinizing solution PR and washing solution WB. Finally the low-salt, high-pH eluent EB elutes pure plasmid DNA from the silica-based membrane.
Product Features
High yield: 0.5-2 mg of pure high-copy plasmid DNA can be rapidly extracted from 150-300 mL of Luria-Bertani culture medium, with an extraction rate of 80-90%;
Stable performance: minimal difference in adsorption capacity between columns, and satisfied repeatability.
Applications
It can be directly used in cell transfection experiments, as well as various molecular biology experiments such as enzyme digestion, transformation, PCR, in vitro transcription, sequencing, etc.
Storage
This kit is stable for 12 months when stored at room temperature. Buffer ER can be transported at room temperature, and stored at 4°C for 1 month and at -20°C for a long time. If the precipitate is produced when stored at 2-8°C, the solution in the kit should be placed at room temperature for a period of time before use, and if necessary, pre-heated in a water bath at 37°C for 10 minutes to dissolve the precipitate. Occasional particles in the reagent do not affect the experimental result.
Prior to the first use, all RNase A from the kit should be added to Buffer P1 (final concentration 100 μg/mL) and stored at 2-8°C.
Product Information
Product Data
According to the results of agarose gel electrophoresis， RK30103 is close to Vendor T
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