Description
This kit uses a modified SDS-alkaline lysis method to lyse cells. The special Buffer P4 and the Filtration Columns 1 can effectively remove impurities such as endotoxin, protein and other impurities.The kit is suitable for extracting plasmid DNA from 5-15 mL bacterial cultures, and the whole experimental procedure of plasmid DNA extraction could be finished within 1 h. Plasmid DNA prepared is suitable for a variety of downstream applications including restriction enzyme digestion, PCR, sequencing, ligation, transformation and cell transfection.
Product Features
- High yield: Up to 70 μg plasmid DNA can be quickly extracted from 5 mL-15 mL bacterial cultures within 1 h.
- High purity: Optimized Buffer is used to reduce endotoxin, protein, SDS and other residues.
- Wide application: Plasmid DNA prepared is suitable for a variety of downstream applications including restriction enzyme digestion, PCR, sequencing, ligation, transformation and cell transfection.
Applications
The plasmid DNA extracted by the kit can be applied to various routine operations, including enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro translation, and transfection of some conventional passaged cells.
Storage
This kit is stable for 12 months when stored at room temperature.
Product Components
| Components | Component number | 50 RXN |
| RNase A (10 mg/mL) | RM30219 | 300 μL |
| Buffer BL2 | RM30211 | 30 mL |
| Buffer P1 | RM30220 | 30 mL |
| Buffer P2 | RM30221 | 30 mL |
| Buffer P4 | RM30214 | 30 mL |
| Buffer DW | RM30222 | 30 mL |
| Buffer DW2 | RM30154 | 12 mL |
| Buffer EB2 | RM30153 | 15 mL |
| Spin Columns 5 | RM30197 | 50 pk |
| Filtration Columns 1 | RM30196 | 50 pk |
| 2 mL Collection Tubes | RM30188 | 2 × 50 pk |
Product Data
- Plasmid extraction was performed from the same volume of bacterial cultures using the RK30105 and Vendor T kits according to their respective instructions. The result showed that the extraction effect of ABclonal RK30105 was better than that of the competing product.
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