AFTSpin EndoFree Plasmid Mini Kit (RK30102)

This kit uses a modified SDS-alkaline lysis method to lyse cells. Crude extract is selectively bound to the spin column by a unique endotoxin scavenger and centrifuged to remove endotoxin. The silicone matrix membrane in the spin column selectively binds plasmid DNA at high salt and low pH conditions, allowing for impurities and other bacterial components to be removed by Buffer PR and Buffer WB. The purified plasmid DNA is eluted from the silicone matrix membrane using low-salt and high-pH Buffer EB.

The plasmid DNA extracted by the kit can be applied to various routine operations, including enzyme digestion, PCR, sequencing, ligation, transformation, library screening, in vitro translation, and transfection of some conventional passaged cells.

This kit is stable for 12 months when stored at room temperature.If the precipitate is produced when stored at 2-8??C, the solution in the kit should be placed at room temperature for a period of time before use, and if necessary, pre-heated in a water bath at 37??C for 10 minutes to dissolve the precipitate. Occasional particles in the reagent do not affect the experimental result. Prior to the first use, all RNase A from the kit should be added to Buffer P1 (final concentration 100 ??g/mL) and stored at 2-8??C.