Description
This kit can be used for rapid extraction of RNA from various trace samples, and does not rely on toxic reagents such as phenol and chloroform. The unique lysis buffer rapidly lyses cells from trace samples and inactivates cell RNA enzymes. The lysate mixture passes through a gDNA Remove Column, where gDNA is removed and RNA is filtered through. After adjusting the binding conditions by ethanol, RNA is selectively bound to the silicon membrane of the adsorption column in the state of high disordered salt. In a series of rapid washing-centrifugation steps, the purified RNA is eluted from the silicon membrane with low salt RNase-free H2O. The extracted RNA can be directly used in RT-PCR, qPCR and RNA library constructing experiments.
Product Features
- Safe: No need to use phenol, chloroform and other toxic reagents.
- Fast: Complete the extraction of several samples within 30 minutes.
- Efficient: The special micro centrifuge column can elute RNA at a minimum volume of 6??L, ensuring the high concentration of extracted RNA.
Applications
Rapid extraction of RNA from various trace samples
Storage
Reagents are stable for 12 months at room temperature.
Product Components
| Components | Component number | Size
50 RXN |
| Buffer RL1 | RM30140 | 30 mL |
| Buffer PR2 | RM30141 | 35 mL |
| RNase-free H2O | RM30142 | 5 mL |
| Buffer WB2 | RM30144 | 12 mL |
| Micro RNase-free adsorption column and collection Tubes | RM30208 | 50 pcs |
| Micro gDNA remove column and collection Tubes | RM30209 | 50 pcs |
| Poly Carrier | RM30206 | 200 ??L |
| Micro pestle | RM30207 | 3 pcs |
| 1.5 mL RNase-free centrifuge Tubes | RM30202 | 50 pcs |
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