Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates, using AMPKa1/AMPKa2 Rabbit pAb (A12718) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Human colon using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of MCF7 cells using AMPKa1/AMPKa2 Rabbit pAb (A12718) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | AMPKa1/AMPKa2 Rabbit pAb |
|---|---|
| Catalog No. | A12718 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human PRKAA1/PRKAA2 (NP_006242.5). |
|---|---|
| Sequence | AEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AMPKa1/AMPKa2 |
| Calculated MW | 64kDa/65kDa/62kDa |
| Observed MW | 62kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | MCF7, 293F, HeLa, C6 |
| Cellular location | apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus. |
| Customer validation | WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Ctenopharyngodon idellus) IHC(Rattus norvegicus) IF(Rattus norvegicus) ELISA(Rattus norvegicus) RT-PCR(Rattus norvegicus) WB(Rattus norvegicus) Other(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A12718? Please let us know so that we can cite the reference in this datasheet.
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