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ANGPT1 Rabbit mAb (A3757)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ANGPT1 Rabbit mAb (A3757)

Western blot analysis of various lysates using ANGPT1 Rabbit mAb (A3757) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunohistochemistry - ANGPT1 Rabbit mAb (A3757)

Immunohistochemistry analysis of paraffin-embedded Human colon using ANGPT1 Rabbit mAb (A3757) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameANGPT1 Rabbit mAb
Catalog No.A3757
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0248
This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Mutations in this gene are associated with hereditary angioedema.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human ANGPT1 (Q15389).
SequenceEMEGKHKEELDTLKEEKENLQGLVTRQTYIIQELEKQLNRATTNNSVLQKQQLELMDTVHNLVNLCTKEGVLLKGGKREEEKPFRDCADVYQAGFNKSGIY
Gene ID
Swiss Prot
SynonymsAGP1; AGPT; ANG1; HAE5; AGPT-1; ANGPT1
Calculated MW58kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse lung, Rat kidney
Cellular locationSecreted.

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    ABclonal:Western blot - ANGPT1 Rabbit mAb (A3757)}

    Western blot - ANGPT1 Rabbit mAb (A3757)

    Western blot analysis of various lysates using ANGPT1 Rabbit mAb (A3757) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 3min.
    ABclonal:Immunohistochemistry - ANGPT1 Rabbit mAb (A3757)}

    Immunohistochemistry - ANGPT1 Rabbit mAb (A3757)

    Immunohistochemistry analysis of paraffin-embedded Human colon using ANGPT1 Rabbit mAb (A3757) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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