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ASC/TMS1 Rabbit pAb (A1170)

Reviews (2)Publications (100) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ASC/TMS1 Rabbit pAb (A1170)

Western blot analysis of lysates from Mouse spleen, using ASC/TMS1 Rabbit pAb (A1170) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Human colon using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)

Immunofluorescence analysis of A-549 cells using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)

Immunofluorescence analysis of paraffin-embedded mouse spleen using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)

Immunofluorescence analysis of paraffin-embedded rat spleen using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for ASC/TMS1 Rabbit pAb(A1170)
Review
ABclonal: review for ASC/TMS1 Rabbit pAb(A1170)

Overview

Product nameASC/TMS1 Rabbit pAb
Catalog No.A1170
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-195 of human ASC/TMS1 (NP_037390.2).
SequenceLDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS
Gene ID
Swiss Prot
SynonymsASC; TMS; TMS1; CARD5; TMS-1; ASC/TMS1
Calculated MW22kDa
Observed MW15kDa, 19-25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus.
Customer validation

(Rat brain)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Canis lupus familiaris, Macaca fascicularis)

IF(Streptococcus pneumoniae, Mus musculus)

IP(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

IHC(Mus musculus)

IHC(Rattus norvegicus, Mus musculus, Canis lupus familiaris)

qPCR(Mus musculus)

RT-qPCR(Homo sapiens)

WB(Homo sapiens, Rattus norvegicus)

ELISA(Rattus norvegicus)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - ASC/TMS1 Rabbit pAb (A1170)}

Western blot - ASC/TMS1 Rabbit pAb (A1170)

Western blot analysis of lysates from Mouse spleen, using ASC/TMS1 Rabbit pAb (A1170) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Human colon using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A1170)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)}

Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)

Immunofluorescence analysis of A-549 cells using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)}

Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)

Immunofluorescence analysis of paraffin-embedded mouse spleen using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)}

Immunofluorescence - ASC/TMS1 Rabbit pAb (A1170)

Immunofluorescence analysis of paraffin-embedded rat spleen using ASC/TMS1 Rabbit pAb (A1170) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (2)

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Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)