제품 > 항체 > 다중클론항체(pAb)

ATF4 Rabbit pAb (A18687)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from C2C12 cells, using ATF4 Rabbit pAb (A18687) at 1:500 dilution.C2C12 cells were treated by tunicamycin (2 μg/ml) for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ATF4 Rabbit pAb (A18687)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using ATF4 Rabbit pAb (A18687) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using ATF4 Rabbit pAb (A18687) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ATF4 Rabbit pAb (A18687)

Immunofluorescence analysis of Hep G2 cells using ATF4 Rabbit pAb(A18687) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	ATF4 Rabbit pAb
Catalog No.	A18687
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 260-351 of human ATF4 (NP_001666.2).
Sequence	PYDPPGEKMVAAKVKGEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKEIQYLKDLIEEVRKARGKKRVP
Gene ID
Swiss Prot
Synonyms	CREB2; TXREB; CREB-2; TAXREB67; ATF4
Calculated MW	39kDa
Observed MW	50kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	C2C12 treated by tunicamycin
Cellular location	Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens, Mus musculus, Sus scrofa)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

ATF4 Rabbit pAb images

Western blot - ATF4 Rabbit pAb (A18687)

Immunohistochemistry - ATF4 Rabbit pAb (A18687)

Immunofluorescence - ATF4 Rabbit pAb (A18687)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Mol Med Rep

Application:

IF:

3.4

Species:

Mus musculus

PMID:

33576466

Title:

A large dose of methamphetamine inhibits drug‑evoked synaptic plasticity via ER stress in the hippocampus

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

2.5

Species:

Rattus norvegicus

PMID:

36244114

Title:

Anti-polycystic ovary syndrome effect of electroacupuncture: IMD inhibits ER stress-mediated apoptosis and autophagy in granulosa cells

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Experimental cell research

Application:

IF:

3.3

Species:

Homo sapiens

PMID:

36209899

Title:

IRE1α-XBP1 regulates PDK1-dependent induction of epithelial-mesenchymal transition in non-small cell lung cancer cells

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

Species:

Homo sapiens

PMID:

36377873

Title:

Upregulation of ATF4-LAMP3 Axis by ORF45 Facilitates Lytic Replication of Kaposi's Sarcoma-Associated Herpesvirus

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

37019162

Title:

Interaction of Bmal1 and eIF2α/ATF4 pathway was involved in Shuxie compound alleviation of circadian rhythm disturbance-induced hepatic endoplasmic reticulum …

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Cell metabolism

Application:

IF:

27.7

Species:

Homo sapiens, Mus musculus

PMID:

35921818

Title:

Aberrant iron distribution via hepatocyte-stellate cell axis drives liver lipogenesis and fibrosis

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Journal of animal science and biotechnology

Application:

IF:

6.3

Species:

Sus scrofa

PMID:

38584279

Title:

Dietary fat supplementation relieves cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis in pigs - PMC

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Bioscience, biotechnology, and biochemistry

Application:

IF:

1.4

Species:

Rattus norvegicus

PMID:

34601561

Title:

SMYD1 alleviates septic myocardial injury by inhibiting endoplasmic reticulum stress

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Biology

Application:

IF:

4.2

Species:

Mus musculus

PMID:

38392333

Title:

Effect of CB1 Receptor Deficiency on Mitochondrial Quality Control Pathways in Gastrocnemius Muscle - PMC

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Oncology letters

Application:

IF:

2.5

Species:

Homo sapiens

PMID:

38846431

Title:

Dihydroartemisinin induces ferroptosis in T cell acute lymphoblastic leukemia cells by downregulating SLC7A11 and activating the ATF4-CHOP signaling pathway - PMC

References for ATF4 Rabbit pAb (A18687)

Product:

ATF4 Rabbit pAb

Journal:

Biochemical and biophysical research communications

Application:

IF:

2.5

Species:

Mus musculus

PMID:

38996786