제품 > 항체 > 다중클론항체(pAb)

ATG7 Rabbit pAb (A0691)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ATG7 Rabbit pAb (A0691) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon using ATG7 Rabbit pAb (A0691) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse lung using ATG7 Rabbit pAb (A0691) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat ovary using ATG7 Rabbit pAb (A0691) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat spleen using ATG7 Rabbit pAb (A0691) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ATG7 Rabbit pAb (A0691)

Immunofluorescence analysis of A-549 cells using ATG7 Rabbit pAb (A0691) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ATG7 Rabbit pAb (A0691) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	ATG7 Rabbit pAb
Catalog No.	A0691
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7 (NP_006386.1).
Sequence	LGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEREGFNFLAKVFNSSHSFL
Gene ID
Swiss Prot
Synonyms	GSA7; APG7L; SCAR31; APG7-LIKE; ATG7
Calculated MW	78kDa
Observed MW	78kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Immunohistochemistry Immunofluorescence
Positive samples	Jurkat, Rat testis
Cellular location	Cytoplasm, Preautophagosomal structure.
Customer validation	(Human colon cancer cell, Human chronic myeloid leukemia cells, U-87MG cells, Mouse adenocarcinoma, Mouse cells, HeLa) WB(Mus musculus, Danio rerio, Homo sapiens, Gallus gallus, Sus scrofa , Rattus norvegicus, Sus scrofa) IF(Mus musculus, Sus scrofa) IP(Rattus norvegicus)

Documents

Certificate of Compliance

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Research Area

ATG7 Rabbit pAb images

Immunohistochemistry - ATG7 Rabbit pAb (A0691)

Immunofluorescence - ATG7 Rabbit pAb (A0691)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Reproductive biology

Application:

IF:

2.376

Species:

Mus musculus

PMID:

31285134

Title:

Heat exposure affected the reproductive performance of pregnant mice: Enhancement of autophagy and alteration of subcellular structure in the corpus luteum.

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Autophagy

Application:

IF:

16.016

Species:

Mus musculus

PMID:

31448672

Title:

PAQR3 suppresses the growth of non-small cell lung cancer cells via modulation of EGFR-mediated autophagy.

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Autophagy

Application:

IF:

16.016

Species:

Danio rerio

PMID:

30205735

Title:

CERKL regulates autophagy via the NAD-dependent deacetylase SIRT1

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Biomedicine & Pharmacotherapy

Application:

IF:

6.529

Species:

Mus musculus

PMID:

31918268

Title:

MiRNA-192-5p attenuates airway remodeling and autophagy in asthma by targeting MMP-16 and ATG7

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Frontiers in Cellular and Infection Microbiology

Application:

IF:

5.293

Species:

Sus scrofa

PMID:

31106159

Title:

Autophagy Is a Defense Mechanism Inhibiting Invasion and Inflammation During High-Virulent Haemophilus parasuis Infection in PK-15 Cells.

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

2020 Jul Chin J Nat Med

Application:

IF:

2.343

Species:

Homo sapiens

PMID:

32616189

Title:

GL-V9 reverses adriamycin resistance in hepatocellular carcinoma cells by affecting JNK2-related autophagy

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Cell Prolif

Application:

IF:

6.83

Species:

Mus musculus

PMID:

33963627

Title:

Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Cell Mol Gastroenterol Hepatol

Application:

IF:

9.225

Species:

Mus musculus

PMID:

33075563

Title:

S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Free radical biology & medicine

Application:

IF:

8.1

Species:

Gallus gallus

PMID:

35675856

Title:

Mercuric chloride induces sequential activation of ferroptosis and necroptosis in chicken embryo kidney cells by triggering ferritinophagy

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

7.46

Species:

Sus scrofa

PMID:

36745686

Title:

Innate sensing of picornavirus infection involves cGAS-STING-mediated antiviral responses triggered by mitochondrial DNA release

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Technology and health care : official journal of the European Society for Engineering and Medicine

Application:

IF:

1.2

Species:

Rattus norvegicus

PMID:

37038787

Title:

Effects of Prunus Tomentosa Thumb Total Flavones on adjuvant arthritis in rats and regulation of autophagy - PMC

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Psychopharmacology

Application:

IF:

4.41

Species:

Rattus norvegicus

PMID:

36949340

Title:

Curcumin protects against doxorubicin induced oxidative stress by regulating the Keap1-Nrf2-ARE and autophagy signaling pathways

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Respiratory research

Application:

IF:

7.16

Species:

Homo sapiens

PMID:

35986277

Title:

Knockdown of HSP110 attenuates hypoxia-induced pulmonary hypertension in mice through suppression of YAP/TAZ-TEAD4 pathway - PMC

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb

Journal:

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)

Application:

IF:

2.24

Species:

Mus musculus

PMID:

37357660

Title:

Autophagy gene Atg7 regulates the development of radiationinduced skin injury and fibrosis of skin

References for ATG7 Rabbit pAb (A0691)

Product:

ATG7 Rabbit pAb