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Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Review (1)Publications (71) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Acetyl-Histone H3-K27 Rabbit pAb
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at 1:2000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at 1:2000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded Human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG(AC005).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.

Review
ABclonal: review for Acetyl-Histone H3-K27 Rabbit pAb(A7253)

Overview

Product nameAcetyl-Histone H3-K27 Rabbit pAb
Catalog No.A7253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Immunogen A synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1).
SequenceMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Gene ID
Swiss Prot
SynonymsH3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K27
Calculated MW15kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
  • ChIP-seq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesNIH/3T3 treated by TSA, C6 treated by TSA
Cellular locationChromosome, Nucleus.
Customer validation

(Prawn gills)

IHC(Rattus norvegicus, Homo sapiens)

IP(Rattus norvegicus, Homo sapiens)

WB(Rattus norvegicus, Danio rerio, Sus scrofa, Mus musculus, Homo sapiens, Monascus ruber, Oryza sativa Japonica Group, Eurosta solidaginis, Drosophila melanogaster, Other, Felis catus)

IF(Homo sapiens, Mus musculus)

IP(Rattus norvegicus)

CUT&Tag(Homo sapiens, Other)

PCR(Homo sapiens)

IF(Homo sapiens)

ChIP(Homo sapiens, Mus musculus, Rattus norvegicus, Oryza sativa, Gallus gallus, Other, Arabidopsis thaliana)

WB(Homo sapiens, Other)

Co-IP(Rattus norvegicus)

ChIP-Seq(Zea mays, Homo sapiens, Other)

ChIP(Other)

Cloning(Mus musculus)

Co-IP(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at 1:2000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at 1:2000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry analysis of paraffin-embedded Human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry analysis of paraffin-embedded Mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry analysis of paraffin-embedded Rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG(AC005).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

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