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Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Dot Blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:3000 dilution.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Immunofluorescence analysis of C6 TSA and C6 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Immunofluorescence analysis of HeLa TSA and HeLa cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Immunofluorescence analysis of NIH-3T3 TSA and NIH-3T3 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Immunoprecipitation analysis of 600ug extracts of HeLa TSA cells using 5ug Acetyl-Histone H3-K4 Rabbit mAb (A24340). Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at a dilition of 1:1000.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody (A24340) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameAcetyl-Histone H3-K4 Rabbit mAb
Catalog No.A24340
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62521
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic acetylated peptide around K4 of human Histone H3 (NP_003520.1).
SequenceARTK(AC)QTARKSTGGK-C
Gene ID
Swiss Prot
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A; Acetyl-Histone H3-K4
Calculated MW16kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • DB 1:2000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa treated by TSA, NIH/3T3 treated by TSA, C6 treated by TSA
Cellular locationChromosome, nucleus.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Western blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Dot Blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Dot Blot - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:3000 dilution.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Immunofluorescence analysis of C6 TSA and C6 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Immunofluorescence analysis of HeLa TSA and HeLa cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Immunofluorescence - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Immunofluorescence analysis of NIH-3T3 TSA and NIH-3T3 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunoprecipitation - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Immunoprecipitation - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Immunoprecipitation analysis of 600ug extracts of HeLa TSA cells using 5ug Acetyl-Histone H3-K4 Rabbit mAb (A24340). Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at a dilition of 1:1000.
    ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K4 Rabbit mAb (A24340)}

    Chromatin Immunoprecipitation - Acetyl-Histone H3-K4 Rabbit mAb (A24340)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody (A24340) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.