제품 > 항체 > 아세틸화 특이적 항체

Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Publications (74) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Acetyl-Histone H3-K9 Rabbit pAb
ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9 antibody (A7255) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameAcetyl-Histone H3-K9 Rabbit pAb
Catalog No.A7255
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H3 (NP_003520.1).
SequenceMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Gene ID
Swiss Prot
SynonymsH3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K9
Calculated MW16kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
  • ChIP-seq 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa treated by TSA, NIH/3T3 treated by TSA, C6 treated by TSA
Cellular locationChromosome, Nucleus.
Customer validation

IHC(Rattus norvegicus)

IP(Rattus norvegicus, Homo sapiens)

WB(Rattus norvegicus, Homo sapiens, Bombyx mori Linnaeus, Mus musculus, Sus scrofa, Saccharomyces cerevisiae, Mus musculus, Rattus norvegicus, Danio rerio, Other, Oryza sativa, Nicotiana benthamiana, Felis catus)

IP(Mus musculus, Rattus norvegicus)

IF(Sus scrofa, Mus musculus, Homo sapiens, Rattus norvegicus)

ChIP(Homo sapiens, Oryza sativa, Other)

ChIP(Homo sapiens, Rattus norvegicus, Mus musculus, Arabidopsis thaliana)

WB(Homo sapiens, Other)

CUT&Tag(Other)

Co-IP(Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.
    ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 Rabbit pAb (A7255)}

    Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9 antibody (A7255) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A7255? Please let us know so that we can cite the reference in this datasheet.

    항체 (105)

    Secondary Antibodies (26)