Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from C6 cells, using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 Rabbit pAb (A7255) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.
Product name | Acetyl-Histone H3-K9 Rabbit pAb |
---|---|
Catalog No. | A7255 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H3 (NP_003520.1). |
---|---|
Sequence | MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY |
Gene ID | |
Swiss Prot | |
Synonyms | H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K9 |
Calculated MW | 16kDa |
Observed MW | 17kDa |
Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) |
---|---|
Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
Recommended dilution |
|
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Key application | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
Positive samples | HeLa treated by TSA, NIH/3T3 treated by TSA, C6 treated by TSA |
Cellular location | Chromosome, Nucleus. |
Customer validation | IHC(Rattus norvegicus) IP(Rattus norvegicus, Homo sapiens) WB(Rattus norvegicus, Homo sapiens, Bombyx mori Linnaeus, Mus musculus, Sus scrofa, Saccharomyces cerevisiae, Mus musculus, Rattus norvegicus, Danio rerio, Other, Oryza sativa, Nicotiana benthamiana, Felis catus) IP(Mus musculus, Rattus norvegicus) IF(Sus scrofa, Mus musculus, Homo sapiens, Rattus norvegicus) ChIP(Homo sapiens, Oryza sativa, Other) ChIP(Homo sapiens, Rattus norvegicus, Mus musculus, Arabidopsis thaliana) WB(Homo sapiens, Other) CUT&Tag(Other) Co-IP(Rattus norvegicus, Mus musculus) |
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Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A7255? Please let us know so that we can cite the reference in this datasheet.
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K4 Rabbit pAb
A2357
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K9 Rabbit pAb
A2360
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K14 Rabbit pAb
A7254
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K18 Rabbit pAb
A7257
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K56 Rabbit pAb
A7256
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb
A2421
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K27 Rabbit pAb
A2361
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S10 Rabbit pAb
AP0840
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit pAb
A2364
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K79 Rabbit pAb
A2369
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R8 Rabbit pAb
A2374
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb
A3155
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K36 Rabbit mAb
A20379
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R2 Rabbit pAb
A2373
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4 Rabbit pAb
A16078
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K27 Rabbit mAb
A2771
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S10 Rabbit mAb
AP0002
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
A17917
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K18 Rabbit pAb
A18807
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K4 Rabbit mAb
A22078
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R26 Rabbit pAb
A3163
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R2 Rabbit pAb
A3154
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R26 Rabbit pAb
A2375
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4 Rabbit pAb
A17019
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K36 Rabbit pAb
A16077
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K14 Rabbit pAb
A18808
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T45 Rabbit pAb
AP0898
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K27 Rabbit pAb
A18825
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit pAb
A20566
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K79 Rabbit pAb
A20822
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K36 Rabbit mAb
A22087
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K4 Rabbit mAb
A22225
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit mAb
A22863
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K27 Rabbit mAb
A22396
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K9 Rabbit mAb
A26196
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K23 Rabbit mAb
A2770
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R8 Rabbit pAb
A3156
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T11 Rabbit pAb
AP0093
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K56 Rabbit pAb
A7262
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K64 Rabbit pAb
A7259
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K36 Rabbit mAb
A22221
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K36 Rabbit mAb
A23944
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
DiMethyl-Histone H3-K14 Rabbit pAb
A5278
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K14 Rabbit pAb
A5279
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K14 Rabbit pAb
A5277
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R8 Rabbit pAb
A3157
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R26 Rabbit pAb
A3153
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R17 Rabbit pAb
A3151
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R17 Rabbit pAb
A3152
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb
A20732
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K27 Rabbit mAb
A22006
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R17 Rabbit mAb
A22291
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
A21299
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S28 Rabbit pAb
AP0839
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T3 Rabbit pAb
AP0846
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S10/T11 Rabbit pAb
AP0896
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T32 Rabbit pAb
AP0897
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-T6 Rabbit pAb
AP0899
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K23 Rabbit pAb
A18154
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-R2 Rabbit mAb
A19645
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb
A17899
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3.3-T3 Rabbit mAb
AP1152
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K36 Rabbit pAb
A20185
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K27 Rabbit pAb
A20184
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R8 Rabbit pAb
A20820
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K79 Rabbit pAb
A20821
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Lactic acid-Histone H3-K18 Rabbit mAb
A21214
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Symmetric DiMethyl-Histone H3-R8 Rabbit mAb
A21207
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K79 Rabbit mAb
A21223
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb
A22144
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K9 Rabbit mAb
A22079
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
MonoMethyl-Histone H3-K27 Rabbit mAb
A22170
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
TriMethyl-Histone H3-K4 Rabbit mAb
A22224
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Rabbit pAb
A21295
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K4 Rabbit mAb
A24341
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Acetyl-Histone H3-K18 Rabbit mAb
A22566
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)
Phospho-Histone H3-S28 Rabbit mAb
AP1431
Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)