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Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at 1:8000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Western blot analysis of lysates from C2C12 cells, using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at 1:8000 dilution. C2C12 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Western blot analysis of lysates from C6 cells, using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at 1:8000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Dot Blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K16 antibody (A23091) at 1:500 dilution.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K16 antibody (A23091) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameAcetyl-Histone H4-K16 Rabbit mAb
Catalog No.A23091
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55790
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
ImmunogenA synthetic acetylated peptide around K16 of human Histone H4 (NP_003529.1).
SequenceMSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYG
Gene ID
Swiss Prot
SynonymsH4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4A; Acetyl-Histone H4-K16
Calculated MW11kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000-1:9000
  • DB 1:100 - 1:500
  • IHC-P 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa treated by TSA, C2C12 treated by TSA, C6 treated by TSA
Cellular locationChromosome, Nucleus.
Customer validation

WB(Homo sapiens)

IP(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at 1:8000 dilution. HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Western blot analysis of lysates from C2C12 cells, using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at 1:8000 dilution. C2C12 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Western blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Western blot analysis of lysates from C6 cells, using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at 1:8000 dilution. C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Dot Blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Dot Blot - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Dot-blot analysis of all sorts of peptides using Acetyl-Histone H4-K16 antibody (A23091) at 1:500 dilution.
    ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Immunohistochemistry - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Acetyl-Histone H4-K16 Rabbit mAb (A23091) at a dilution of 1:1200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H4-K16 Rabbit mAb (A23091)}

    Chromatin Immunoprecipitation - Acetyl-Histone H4-K16 Rabbit mAb (A23091)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K16 antibody (A23091) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A23091? Please let us know so that we can cite the reference in this datasheet.

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