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Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)

Western blot analysis of lysates from HeLa cells, using Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069) at 1:500 dilution.HeLa cells were treated by Sodium butyrate (5 mM) for 16 hours after serum-starvation 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)

Western blot analysis of various lysates, using Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)

Immunofluorescence analysis of paraffin-embedded rat testis using Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameAcetyl-alpha Tubulin -K40 Rabbit pAb
Catalog No.A23069
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic acetylated peptide around K40 of human alpha Tubulin.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsLIS3; TUBA3; B-ALPHA-1; Acetyl-alpha Tubulin -K40
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa treated by Sodium butyrate, Mouse testis, Rat testis
Cellular location

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ABclonal:Western blot - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)}

Western blot - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)

Western blot analysis of lysates from HeLa cells, using Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069) at 1:500 dilution.HeLa cells were treated by Sodium butyrate (5 mM) for 16 hours after serum-starvation 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)}

Western blot - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)

Western blot analysis of various lysates, using Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)}

Immunofluorescence - Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069)

Immunofluorescence analysis of paraffin-embedded rat testis using Acetyl-alpha Tubulin -K40 Rabbit pAb (A23069) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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