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β-Actin Mouse mAb (AC004)

Reviews (2)Publications (390) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:β-Actin Mouse mAb
ABclonal:Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of lysates from HeLa cells, using β-Actin Mouse mAb (AC004) at 1:2000- 1:640000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of various lysates, using β-Actin Mouse mAb (AC004) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - β-Actin Mouse mAb (AC004)

Immunofluorescence analysis of U2OS cells using β-Actin Mouse mAb (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for β-Actin Mouse mAb(AC004)
Review
ABclonal: review for β-Actin Mouse mAb(AC004)

Overview

Product nameβ-Actin Mouse mAb
Catalog No.AC004
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1, Kappa
CloneNo.AMC0001
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
ImmunogenFusion protein of human β-Actin
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsBRWS1; PS1TP5BP1; β-Actin
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000-1:160000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Antibody Dilute ProcedurePut 5ul AC004 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HeLa, A-549, NIH3T3, Mouse brain, Rat testis, C6
Cellular locationCytoplasm, cytoskeleton.
Customer validation

(Mouse kidney, Rat kidney, Homo sapiens)

WB(Mus musculus, Homo sapiens, Crassostrea gigas, Rattus norvegicus, Citrus sinensis, Sus scrofa, Ctenopharyngodon idellus, Cynoglossus semilaevis, Spodoptera frugiperda, Pinellia ternata, Arabidopsis thaliana, Actinopterygii, Bombyx mori Linnaeus, Lineage, Canis lupus familiaris, Lampetra japonica, Staphylococcus aureus, Rattusnorvegicus, Mus musculus, Rattus norvegicus, Gallus gallus, Danio rerio, Larimichthys crocea, Other, Ictalurus punctatus, Bos taurus, Oryza sativa, Caenorhabditis elegans, Drosophila melanogaster)

IF(Homo sapiens, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

IP(Homo sapiens)

Co-IP(Homo sapiens)

IHC(Mus musculus)

Other(Mus musculus)

qPCR(Mus musculus)

IHC(Mus musculus)

IP(mus musculus, Homo sapiens)

ChIP(mus musculus)

Co-IP(Homo sapiens)

WB(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - β-Actin Mouse mAb (AC004)}

Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of lysates from HeLa cells, using β-Actin Mouse mAb (AC004) at 1:2000- 1:640000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - β-Actin Mouse mAb (AC004)}

Western blot - β-Actin Mouse mAb (AC004)

Western blot analysis of various lysates, using β-Actin Mouse mAb (AC004) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)}

Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)}

Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Mouse mAb (AC004)}

Immunohistochemistry - β-Actin Mouse mAb (AC004)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - β-Actin Mouse mAb (AC004)}

Immunofluorescence - β-Actin Mouse mAb (AC004)

Immunofluorescence analysis of U2OS cells using β-Actin Mouse mAb (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

재조합 단백질 (1)

ELISA 키트 (1)

Secondary Antibodies (22)