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β-Actin Rabbit mAb (AC038)

Publications (181) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

ABclonal:Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of various lysates using β-Actin Rabbit mAb (AC038) at 1:50000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of lysates from HeLa cells, using β-Actin Rabbit mAb (AC038) at 1:10000-1:320000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of various lysates using β-Actin Rabbit mAb (AC038) at 1:50000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of lysates from HeLa cells using β-Actin Rabbit mAb (AC038) at 1:10000-1:160000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Rat spleen_ tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Overview

Product nameβ-Actin Rabbit mAb
Catalog No.AC038
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5115-01
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
ImmunogenRecombinant protein of human β-Actin
SequenceMDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID
Swiss Prot
SynonymsBRWS1; PS1TP5BP1; β-Actin
Calculated MW42kDa
Observed MW45kDa/42kDa/42kDa
ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:100000
  • IHC-P 1:500 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, HeLa, Mouse brain, Mouse testis, Mouse lung, Rat brain, Rat testis, Rat lung, 293F, HeLa, Hep G2, NIH/3T3, C6, Mouse brain, Rat lung, HeLa
Cellular locationCytoplasm, cytoskeleton.
Customer validation

WB(Sanghuangporus vaninii, Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Bacillus cereus, Mus musculus, A. fumigatus strains, Rattus norvegicus, Danio rerio, Other)

IP(Mus musculus)

IF(mus musculus)

IF(Mus musculus)

RT-PCR(Homo sapiens, Rattus norvegicus)

IHC(Homo sapiens)

RIP(Homo sapiens)

RT-PCR(Homo sapiens)

ELISA(Mus musculus)

Co-IP(Homo sapiens)

IF(Sus scrofa, Homo sapiens, Mus musculus)

qPCR(Mus musculus)

PCR(Mus musculus)

RT-qPCR(Mus musculus)

Other(Homo sapiens, Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - β-Actin Rabbit mAb (AC038)}

Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of various lysates using β-Actin Rabbit mAb (AC038) at 1:50000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - β-Actin Rabbit mAb (AC038)}

Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of lysates from HeLa cells, using β-Actin Rabbit mAb (AC038) at 1:10000-1:320000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - β-Actin Rabbit mAb (AC038)}

Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of various lysates using β-Actin Rabbit mAb (AC038) at 1:50000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - β-Actin Rabbit mAb (AC038)}

Western blot - β-Actin Rabbit mAb (AC038)

Western blot analysis of lysates from HeLa cells using β-Actin Rabbit mAb (AC038) at 1:10000-1:160000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)}

Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)}

Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)}

Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (AC038)}

Immunohistochemistry - β-Actin Rabbit mAb (AC038)

Immunohistochemistry analysis of paraffin-embedded Rat spleen_ tissue using β-Actin Rabbit mAb (AC038) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using AC038? Please let us know so that we can cite the reference in this datasheet.

항체 (6)

β-Actin Rabbit mAb (High Dilution)
AC026

WBIHC-PELISA

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

β-Actin Rabbit mAb (High Dilution)

β-Actin Mouse mAb
AC004

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

β-Actin Mouse mAb

β-Actin Rabbit pAb
AC006

WB

Reactivity:Human, Mouse, Rat

β-Actin Rabbit pAb

HRP-conjugated β-Actin Rabbit mAb
AC028

WBELISA

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

HRP-conjugated β-Actin Rabbit mAb

HRP-conjugated β-Actin Mouse mAb
AC043

WBELISA

Reactivity:Human, Mouse, Rat

HRP-conjugated β-Actin Mouse mAb

β-Actin Rabbit mAb
AC050

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

β-Actin Rabbit mAb

재조합 단백질 (1)

Recombinant Human Beta-actin/ACTB Protein
RP02968LQ

Tags:C-His

Expressed Host:E. coli

Recombinant Human Beta-actin/ACTB Protein

ELISA 키트 (1)

Human β-actin ELISA Kit
RK10200

Human β-actin ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)