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β-Actin Rabbit mAb (High Dilution) (AC026)

Reviews (6)Publications (2166) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

ABclonal:β-Actin Rabbit mAb (High Dilution)
ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of various lysates using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:100000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of lysates from HeLa cells, using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)

Overview

Product nameβ-Actin Rabbit mAb (High Dilution)
Catalog No.AC026
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC5115-01
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Note：Due to the high antibody titer, it is advisable to be diluted before use. Please dilute 5μL of the antibody solution with 45μL of PBS solution, containing 50% glycerol. The diluted antibody can be stored at -20°C.
ImmunogenRecombinant protein of human β-actin
SequenceMDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID
Swiss Prot
SynonymsBRWS1; PS1TP5BP1; β-Actin
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:80000-1:640000
  • IHC-P 1:100 - 1:500
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Antibody Dilute ProcedurePut 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azid, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesTE-1, SW480, NIH/3T3, 293T, Mouse brain, Mouse testis, mose lung, rat brain
Cellular locationCytoplasm, cytoskeleton.
Customer validation

(BV2 cells, HeLa treated by Starvatio and IGF, rat brain cells, Pig lung, 293T, DF-1, Mus musculus)

WB(Mus musculus, Rattus norvegicus, Mactra, Gallus gallus, Homo sapiens, Bos taurus, Sus scrofa, Danio rerio, Zea mays, Cynoglossus semilaevis, Mycobacterium tuberculosis, Cavia porcellus, Macaca mulatta, Arabidopsis thaliana, Malus micromalus, Cyprinus carpio, Alpinia katsumadai Hayata, Helicoverpa armigera, Anatinae, Oreochroms mossambcus, Cricetinae, Edwardsiella tarda, Drosophila melanogaster, Mauremys sinensis , Ictalurus punctatus , Lotus japonicus, Meretrix petechialis, Ctenopharyngodon, Eospalax cansus, Cotton bollworms, Ctenopharyngodon idellus, Echinococcus multilocularis, Nicotiana benthamiana, Vaccinium dunalianum, Caenorhabditis elegans, Escherichia coli, Hordeum vulgare, Haliotis, Mus musculus, Carassius auratus gibelio, Capra hircus, Chlorocebus aethiops, Megalobrama amblycephala, Mancinella armigera, Anser cygnoides domesticus, Puerariae Flos, Felis catus, Ctenopharyngodon idella, Oreochromis mossambicus, Nicotiana tabacum, Scylla, Mesocricetus auratus, Rattus, Cricetulus migratorius, Pampus argenteus, Locusta migratoria, Pelteobagrus fulvidraco, Anser albifrons , Micropterus salmoides, Oreochromis niloticus, Trichopodus trichopterus, Rattus norvegicus, Oryctolagus cuniculus, Acanthopagrus schlegelii, Mytilus coruscus , Mus musculusRattus norvegicus, Fathead minnow, King Charles Spaniel, Pimephales promelas, Dianella ensifolia, Chlorocebus aethiops, Spondyliosoma cantharus, Other, Ovis aries, Plutella xylostella, Squaliobarbus curriculus, Sus scrofa, Bos taurus, Oryza sativa, Takifugu rubripes, Bombyx mori Linnaeus)

IHC(Mus musculus, Homo sapiens, Oryctolagus cuniculus)

(Mus musculus)

IP(Other, Danio rerio, Homo sapiens, Mus musculus)

IHC(Homo sapiens,Rattus norvegicus, Homo sapiens, Mus musculus, Chlorocebus aethiops)

IF(Homo sapiens, Danio rerio, Mus musculus)

qRT-PCR(Gallus gallus)

IHC(Homo sapiens)

IP(Homo sapiens, Rattus norvegicus)

IP(Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus, Sus scrofa, Rattus norvegicus, Bos taurus)

DB(Mus musculus)

Co-IP(Homo sapiens, Zea mays, Other, Mus musculus)

ELISA(Mus musculus, Rattus norvegicus)

WB(Mus musculus)

qPCR(Mus musculus, Homo sapiens, Cyprinus carpio)

ChIP(Other)

WB(Other, Zea mays, Homo sapiens, Gallus gallus, Mus musculus, Rattus norvegicus)

FC(Sus scrofa)

Co-IP(Mus musculus)

Other(Mus musculus, Oryctolagus cuniculus, Zea mays)

RT-PCR(Homo sapiens)

qPCR(Gallus gallus)

RNA-Seq(Mus musculus)

RT-qPCR(Mus musculus)

IF(Homo sapiens, Rattus norvegicus)

Enzyme assay(Danio rerio)

PCR(Rattus norvegicus, Mus musculus)

Other(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)}

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of various lysates using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:100000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)}

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of lysates from HeLa cells, using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)}

Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)}

Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)}

Immunohistochemistry - β-Actin Rabbit mAb (High Dilution) (AC026)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using β-Actin Rabbit mAb (High Dilution) (AC026) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for AC026

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Reviews List

Publishing research using AC026? Please let us know so that we can cite the reference in this datasheet.

항체 (6)

β-Actin Mouse mAb
AC004

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

β-Actin Mouse mAb

β-Actin Rabbit mAb
AC038

WBIHC-PELISA

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

β-Actin Rabbit mAb

β-Actin Rabbit pAb
AC006

WB

Reactivity:Human, Mouse, Rat

β-Actin Rabbit pAb

HRP-conjugated β-Actin Rabbit mAb
AC028

WBELISA

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

HRP-conjugated β-Actin Rabbit mAb

HRP-conjugated β-Actin Mouse mAb
AC043

WBELISA

Reactivity:Human, Mouse, Rat

HRP-conjugated β-Actin Mouse mAb

β-Actin Rabbit mAb
AC050

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

β-Actin Rabbit mAb

재조합 단백질 (1)

Recombinant Human Beta-actin/ACTB Protein
RP02968LQ

Tags:C-His

Expressed Host:E. coli

Recombinant Human Beta-actin/ACTB Protein

ELISA 키트 (1)

Human β-actin ELISA Kit
RK10200

Human β-actin ELISA Kit

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)