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β-Actin Rabbit pAb (AC006)

Review (1)Publications (149) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:β-Actin Rabbit pAb
ABclonal:Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of various lysates using β-Actin Rabbit pAb (AC006) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for β-Actin Rabbit pAb(AC006)

Overview

Product nameβ-Actin Rabbit pAb
Catalog No.AC006
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human β-Actin (NP_001092.1).
SequenceMDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID
Swiss Prot
SynonymsBRWS1; PS1TP5BP1; β-Actin
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, MCF7, A-431, NIH/3T3, C6, Mouse testis, Mouse kidney, Rat testis
Cellular locationCytoplasm, cytoskeleton.
Customer validation

(MSC cells)

WB(Homo sapiens, Crassostrea gigas, Rattus norvegicus, Mus musculus, Sus scrofa, Arabidopsis thaliana, Gallus gallus, flounder, Melodinus cochinchinensis, Tree, Flowers, Verticillium dahliae, Rattus norvegicus, Mus musculus, Anser cygnoides, Other, Lampetra japonica)

IP(Homo sapiens, Mus musculus)

IF(Homo sapiens, Rattus norvegicus, Homo sapiens, Mus musculus)

IP(Mus musculus)

qRT-PCR(Homo sapiens, Mus musculus)

IF(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - β-Actin Rabbit pAb (AC006)}

Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of various lysates using β-Actin Rabbit pAb (AC006) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (6)

재조합 단백질 (1)

ELISA 키트 (1)

Secondary Antibodies (26)