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Actin (plant specific) Mouse mAb (AC009)

Review (1)Publications (79) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Various Plants

ABclonal:Actin (plant specific) Mouse mAb
ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)

Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (AC009).
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using Actin (plant specific) Mouse mAb (AC009) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Review
ABclonal: review for Actin (plant specific) Mouse mAb(AC009)

Overview

Product nameActin (plant specific) Mouse mAb
Catalog No.AC009
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG1
CloneNo.AMC0496
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
ImmunogenA synthetic peptide corresponding to Actin (plant specific).
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW45kDa
Observed MW45kDa
ReactivityVarious Plants
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesArabidopsis thaliana tissue, Arabidopsis thaliana, Oryza sativa, Cassava, Zea mays
Cellular location
Customer validation

(Zea mays,Arabidopsis thaliana,Tomato)

WB(Brassica napus, Arabidopsis thaliana, Populus L., Homo sapiens, Oryza sativa, N. benthamiana plants, Triticum aestivum, Mus musculus, Zea mays, Solanum tuberosum , Nicotiana benthamiana, Paeonia suffruticosa, Other, Glycine max, Populus trichocarpa Torr. & Gray, Trifoliata orange, Rattus norvegicus, Saccharomyces cerevisiae, Cyprinus carpio, Ctenopharyngodon idellus)

Co-IP(Arabidopsis thaliana, Other)

Other(Arabidopsis thaliana)

IP(Arabidopsis thaliana)

WB(Other)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)}

    Western blot - Actin (plant specific) Mouse mAb (AC009)

    Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb (AC009).
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    ABclonal:Western blot - Actin (plant specific) Mouse mAb (AC009)}

    Western blot - Actin (plant specific) Mouse mAb (AC009)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using Actin (plant specific) Mouse mAb (AC009) at 1:5000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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