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Adenosine Deaminase (ADA) Rabbit pAb (A1019)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

Western blot analysis of various lysates using Adenosine Deaminase (ADA) Rabbit pAb (A1019) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

Immunofluorescence analysis of U2OS cells using Adenosine Deaminase (ADA) Rabbit pAb (A1019) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

Immunofluorescence analysis of MOLT-4 cells using Adenosine Deaminase (ADA) Rabbit pAb (A1019) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameAdenosine Deaminase (ADA) Rabbit pAb
Catalog No.A1019
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-363 of human Adenosine Deaminase (Adenosine Deaminase (ADA)) (NP_000013.2).
SequenceMAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL
Gene ID
Swiss Prot
SynonymsADA1; Adenosine Deaminase (ADA)
Calculated MW41kDa
Observed MW41kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat, Rat lung
Cellular locationCell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle lumen, Extracellular side, Peripheral membrane protein.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Adenosine Deaminase (ADA) Rabbit pAb (A1019)}

    Western blot - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

    Western blot analysis of various lysates using Adenosine Deaminase (ADA) Rabbit pAb (A1019) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019)}

    Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

    Immunofluorescence analysis of U2OS cells using Adenosine Deaminase (ADA) Rabbit pAb (A1019) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019)}

    Immunofluorescence - Adenosine Deaminase (ADA) Rabbit pAb (A1019)

    Immunofluorescence analysis of MOLT-4 cells using Adenosine Deaminase (ADA) Rabbit pAb (A1019) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)