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Affinity Gel-conjugated Rabbit anti Myc-Tag mAb (AE120)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Species independent

ABclonal:Immunoprecipitation - Affinity Gel-conjugated Rabbit anti Myc-Tag mAb {Anti-Myc-Tag 亲和凝胶} (AE120)

Immunoprecipitation of PTEN-Myc from 150 µg extracts of 293T cells transfected with a PTEN expression vector containing a single C-terminal Myc-Tag was performed using 20 µl of Affinity Gel-conjugated Rabbit anti Myc-Tag mAb (AE120). Affinity Gel-conjugated Rabbit IgG isotype control mAb was used to precipitate the Control IgG sample. The IP sample was eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using HRP-conjugated Rabbit anti Myc-Tag mAb (AE102) at a dilution of 1:5000.

Overview

Product nameAffinity Gel-conjugated Rabbit anti Myc-Tag mAb
Catalog No.AE120
Host speciesRabbit
Purification methodAffinity purification
Isotype
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
ImmunogenA synthetic peptide corresponding to Myc tag.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsMyc; Myc tag; Myc-tag; Myc-Tag
Calculated MW
Observed MW54kDa(PTEN)
ReactivitySpecies independent
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IP 20μl-40μl Affinity Gel for 100μg-300μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunoprecipitation    
Positive samples293T-PTEN-MYC-C
Cellular location
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunoprecipitation - Affinity Gel-conjugated Rabbit anti Myc-Tag mAb {Anti-Myc-Tag 亲和凝胶} (AE120)}

    Immunoprecipitation - Affinity Gel-conjugated Rabbit anti Myc-Tag mAb {Anti-Myc-Tag 亲和凝胶} (AE120)

    Immunoprecipitation of PTEN-Myc from 150 µg extracts of 293T cells transfected with a PTEN expression vector containing a single C-terminal Myc-Tag was performed using 20 µl of Affinity Gel-conjugated Rabbit anti Myc-Tag mAb (AE120). Affinity Gel-conjugated Rabbit IgG isotype control mAb was used to precipitate the Control IgG sample. The IP sample was eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using HRP-conjugated Rabbit anti Myc-Tag mAb (AE102) at a dilution of 1:5000.

    * For research use only. Not for therapeutic or diagnostic purposes.

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