Review (1)Publications (7) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
The multiplex IHC analysis on paraffin-embedded Human breast cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : Ki67 Rabbit mAb (A20018, 1:500) with TSA-TYR-520 (Green), HER2/ErbB2 Rabbit mAb (A21248, 1:200) with TSA-TYR-570 (Red), and Androgen Receptor Rabbit mAb (A19611, 1:400) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.
Western blot analysis of lysates from mouse testis, using Androgen Receptor Rabbit mAb (A19611) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human prostate cancer using Androgen Receptor Rabbit mAb (A19611) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human prostate cancer using Androgen Receptor Rabbit mAb (A19611, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunoprecipitation analysis of 600 μg extracts of Mouse testis cells using 3 μg Androgen Receptor antibody (A19611). Western blot was performed from the immunoprecipitate using Androgen Receptor antibody (A19611) at a dilution of 1:1000.
| Product name | Androgen Receptor Rabbit mAb |
|---|---|
| Catalog No. | A19611 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0090 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Androgen Receptor (P10275). |
|---|---|
| Sequence | MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAH |
| Gene ID | |
| Swiss Prot | |
| Synonyms | KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; Androgen Receptor |
| Calculated MW | 99kDa |
| Observed MW | 110kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | Mouse testis |
| Cellular location | Nucleus. |
| Customer validation | (testicular tissue) IF(Mus musculus, Homo sapiens, Mus musculus) WB(Homo sapiens, Mus musculus) WB(Homo sapiens, Mus musculus) IHC(Homo sapiens) WB(Homo sapiens) |
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