제품 > 항체 > 단일클론항체(mAb)

Arginase 1 (ARG1) Rabbit mAb (A4923)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Rat liver, using Arginase 1 (Arginase 1 (ARG1)) Rabbit mAb (A4923) at 1:5000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Arginase 1 (ARG1) Rabbit mAb (A4923)

Immunofluorescence analysis of paraffin-embedded rat liver using Arginase 1 (ARG1) Rabbit mAb (A4923) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal imaging of paraffin-embedded human liver using Arginase 1 (ARG1) Rabbit mAb (A4923, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.

Confocal imaging of paraffin-embedded mouse liver using Arginase 1 (ARG1) Rabbit mAb (A4923, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x.

Overview

Product name	Arginase 1 (ARG1) Rabbit mAb
Catalog No.	A4923
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1164

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Arginase 1 (ARG1) (P05089).
Sequence	MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGD
Gene ID
Swiss Prot
Synonyms	ARG1; arginase-1; Arginase 1 (ARG1)
Calculated MW	35kDa
Observed MW	40kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:5000 - 1:30000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Rat liver
Cellular location	Cytoplasm, nuclear.
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus, Mus musculus) IHC(Sus scrofa, Mus musculus, Rattus norvegicus) IF(Mus musculus, Homo sapiens) IF(Homo sapiens, Rattus norvegicus) WB(Mus musculus) WB (Mus musculus)

Documents

Certificate of Compliance

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Research Area

Arginase 1 (ARG1) Rabbit mAb images

Western blot - Arginase 1 (ARG1) Rabbit mAb (A4923)

Immunofluorescence - Arginase 1 (ARG1) Rabbit mAb (A4923)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

ACS Omega

Application:

IF:

3.7

Species:

Mus musculus

PMID:

33163819

Title:

Impact of Marine-Based Biomaterials on the Immunoregulatory Properties of Bone Marrow-Derived Mesenchymal Stem Cells: Potential Use of Fish Collagen in Bone Tissue Engineering

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Mol Med Rep

Application:

IF:

3.4

Species:

Homo sapiens

PMID:

34080032

Title:

Porcupine inhibitor LGK?974 inhibits Wnt/β?catenin signaling and modifies tumor?associated macrophages resulting in inhibition of the malignant behaviors of non?small cell lung cancer cells

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

International journal of molecular sciences

Application:

IHC

IF:

5.6

Species:

Sus scrofa

PMID:

34948290

Title:

Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Journal of Crohn's & colitis

Application:

IF:

8.3

Species:

Mus musculus

PMID:

35134154

Title:

Tollip orchestrates macrophage polarization to alleviate intestinal mucosal inflammation

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.6

Species:

Rattus norvegicus

PMID:

35658977

Title:

DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Frontiers in medicine

Application:

IHC

IF:

5.1

Species:

Mus musculus

PMID:

36714100

Title:

Cryptotanshinone attenuates LPS-induced acute lung injury by regulating metabolic reprogramming of macrophage - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

36809720

Title:

Dioscin modulates macrophages polarization and MDSCs differentiation to inhibit tumorigenesis of colitis-associated colorectal cancer

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

6.9

Species:

Homo sapiens

PMID:

37236023

Title:

Huoluo Xiaoling Pellet promotes microglia M2 polarization through increasing MCPIP1 expression for ischemia stroke alleviation

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.6

Species:

Mus musculus,Rattus norvegicus

PMID:

37264405

Title:

Microglia-derived TNF-α contributes to RVLM neuronal mitochondrial dysfunction via blocking the AMPK-Sirt3 pathway in stress-induced hypertension - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Cells

Application:

IF:

7.7

Species:

Mus musculus

PMID:

37443768

Title:

TMEM106B Puncta Is Increased in Multiple Sclerosis Plaques, and Reduced Protein in Mice Results in Delayed Lipid Clearance Following CNS Injury - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Cancer immunology research

Application:

IF:

8.1

Species:

Homo sapiens, Mus musculus

PMID:

36812256

Title:

Netrin-1 promotes the immunosuppressive activity of MDSCs in colorectal cancer

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

iScience

Application:

WB, IF

IF:

5.8

Species:

Homo sapiens

PMID:

37829207

Title:

Dental pulp stem cells accelerate wound healing through CCL2-induced M2 macrophages polarization - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

iScience

Application:

IF:

5.8

Species:

Homo sapiens

PMID:

37829207

Title:

Dental pulp stem cells accelerate wound healing through CCL2-induced M2 macrophages polarization - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Antioxidants (Basel, Switzerland)

Application:

IF:

7.7

Species:

Mus musculus

PMID:

38136199

Title:

DHA and EPA Alleviate Epileptic Depression in PTZ-Treated Young Mice Model by Inhibiting Neuroinflammation through Regulating Microglial M2 Polarization and Improving Mitochondrial Metabolism - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Phytotherapy research : PTR

Application:

IF:

6.1

Species:

Rattus norvegicus

PMID:

37871970

Title:

Stigmasterol regulates microglial M1/M2 polarization via the TLR4/NFB pathway to alleviate neuropathic pain

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Nature communications

Application:

IF:

16.6

Species:

Mus musculus

PMID:

38331933

Title:

Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Particle and fibre toxicology

Application:

IF:

7.2

Species:

Mus musculus

PMID:

38419076

Title:

Zinc oxide nanoparticles exacerbate skin epithelial cell damage by upregulating pro-inflammatory cytokines and exosome secretion in M1 macrophages following UVB irradiation-induced skin injury - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

38457930

Title:

Pentraxin 3 exacerbates psoriasiform dermatitis through regulation of macrophage polarization

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Mus musculus

PMID:

38468126

Title:

Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR2/SRC pathway in vascular cognitive impairment mice

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Molecular biology reports

Application:

IF:

2.6

Species:

Mus musculus

PMID:

38270817

Title:

Sodium humate ameliorates LPS-induced liver injury in mice by inhibiting TLR4/NF-B and activating NRF2/HO-1 signaling pathways

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

ACS nano

Application:

IF:

15.8

Species:

Mus musculus

PMID:

36715347

Title:

Dual-Targeted Nanodiscs Revealing the Cross-Talk between Osteogenic Differentiation of Mesenchymal Stem Cells and Macrophages

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Investigative ophthalmology & visual science

Application:

IF:

Species:

Mus musculus

PMID:

37145604

Title:

Microglial CD11b Knockout Contributes to Axonal Debris Clearance and Axonal Degradation Attenuation via IGF-1 After Acute Optic Nerve Injury

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Application:

WB, IF

IF:

4.8

Species:

Rattus norvegicus

PMID:

37534961

Title:

Neutrophil extracellular traps facilitate sympathetic hyperactivity by polarizing microglia toward M1 phenotype after traumatic brain injury

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Journal of neuroinflammation

Application:

IF, WB

IF:

9.6

Species:

Mus musculus

PMID:

38840180

Title:

CD36 deletion prevents white matter injury by modulating microglia polarization through the Traf5-MAPK signal pathway - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Mus musculus

PMID:

36003390

Title:

EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Journal of nanobiotechnology

Application:

IF:

10.2

Species:

Rattus norvegicus

PMID:

38970101

Title:

Neuroimmune modulating and energy supporting nanozyme-mimic scaffold synergistically promotes axon regeneration after spinal cord injury - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

iScience

Application:

IF:

5.8

Species:

Mus musculus,Rattus norvegicus

PMID:

38638567

Title:

Semaphorin3C identified as mediator of neuroinflammation and microglia polarization after spinal cord injury - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Gut microbes

Application:

IF:

12.2

Species:

Mus musculus

PMID:

39012957

Title:

Gut microbiota promotes macrophage M1 polarization in hepatic sinusoidal obstruction syndrome via regulating intestinal barrier function mediated by butyrate - PMC

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Journal of molecular neuroscience : MN

Application:

IHC

IF:

2.8

Species:

Rattus norvegicus

PMID:

38954245

Title:

The Possible Neuroprotective Effect of Caffeic Acid on Cognitive Changes and Anxiety-Like Behavior Occurring in Young Rats Fed on High-Fat Diet and Exposed to

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Neuroscience

Application:

IF:

2.9

Species:

Rattus norvegicus

PMID:

38782114

Title:

Glycoprotein non-metastatic melanoma protein B (GPNMB) protects against neuroinflammation and neuronal loss in pilocarpine-induced epilepsy via the regulation of

References for Arginase 1 (ARG1) Rabbit mAb (A4923)

Product:

Arginase 1 (ARG1) Rabbit mAb

Journal:

Journal of inflammation research

Application:

IF:

4.5

Species:

Mus musculus

PMID:

39372592