제품 > 항체 > 메틸화 특이적 항체

Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

Western blot analysis of various lysates, using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at1:4000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Dot Blot - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

Dot-blot analysis of all sorts of peptides using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at 1:8200 dilution.

ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

Immunohistochemistry analysis of paraffin-embedded Human colon using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

Immunohistochemistry analysis of paraffin-embedded Human kidney using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameAsymmetric DiMethyl-Histone H3-R8 Rabbit mAb
Catalog No.A22144
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC54909
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic asymmetric dimethylated peptide around R8 of human Histone H3 (NP_003520.1).
SequenceMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAY
Gene ID
Swiss Prot
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A; Asymmetric DiMethyl-Histone H3-R8
Calculated MW16kDa
Observed MW17kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range Predicted)
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:4000
  • DB 1:5000 - 1:8000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, nucleus.
Customer validation

PCR(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)}

    Western blot - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

    Western blot analysis of various lysates, using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at1:4000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Dot Blot - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)}

    Dot Blot - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

    Dot-blot analysis of all sorts of peptides using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at 1:8200 dilution.
    ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)}

    Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

    Immunohistochemistry analysis of paraffin-embedded Rat lung using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)}

    Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

    Immunohistochemistry analysis of paraffin-embedded Mouse lung using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)}

    Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

    Immunohistochemistry analysis of paraffin-embedded Human colon using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)}

    Immunohistochemistry - Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144)

    Immunohistochemistry analysis of paraffin-embedded Human kidney using Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb (A22144) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A22144? Please let us know so that we can cite the reference in this datasheet.

    항체 (105)

    Secondary Antibodies (26)