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BDNF Mouse mAb (A18129)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - BDNF Mouse mAb (A18129)

Western blot analysis of various lysates using BDNF Mouse mAb (A18129) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameBDNF Mouse mAb
Catalog No.A18129
Host speciesMouse
Purification methodAffinity purification
IsotypelgG2a, kappa
CloneNo.AMC0122
This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-247 of human BDNF (P23560).
SequencePMKEANIRGQGGLAYPGVRTHGTLESVNGPKAGSRGLTSLADTFEHVIEELLDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR
Gene ID
Swiss Prot
SynonymsANON2; BULN2; BDNF
Calculated MW28kDa
Observed MW28kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:20000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse lung, Mouse brain, Rat lung
Cellular locationSecreted.
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - BDNF Mouse mAb (A18129)}

Western blot - BDNF Mouse mAb (A18129)

Western blot analysis of various lysates using BDNF Mouse mAb (A18129) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18129? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (22)