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BRCA2 Rabbit pAb (A2435)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BRCA2 Rabbit pAb (A2435)

Western blot analysis of lysates from Mouse liver, using BRCA2 Rabbit pAb (A2435) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BRCA2 Rabbit pAb (A2435)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using BRCA2 Rabbit pAb (A2435) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BRCA2 Rabbit pAb (A2435)

Immunohistochemistry analysis of paraffin-embedded Human lung tissue using BRCA2 Rabbit pAb (A2435) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - BRCA2 Rabbit pAb (A2435)

Immunofluorescence analysis of C6 cells using BRCA2 Rabbit pAb (A2435) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBRCA2 Rabbit pAb
Catalog No.A2435
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The largest exon in both genes is exon 11, which harbors the most important and frequent mutations in breast cancer patients. The BRCA2 gene was found on chromosome 13q12.3 in human. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1400-1600 of BRCA2 (NP_000050.3).
SequenceKESFNKIVNFFDQKPEELHNFSLNSELHSDIRKNKMDILSYEETDIVKHKILKESVPVGTGNQLVTFQGQPERDEKIKEPTLLGFHTASGKKVKIAKESLDKVKNLFDEKEQGTSEITSFSHQWAKTLKYREACKDLELACETIEITAAPKCKEMQNSLNN
Gene ID
Swiss Prot
SynonymsFAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2; BRCA2
Calculated MW384kDa
Observed MW380kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Customer validation

IF(Oryza sativa)

WB(Homo sapiens)

Co-IP(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - BRCA2 Rabbit pAb (A2435)}

Western blot - BRCA2 Rabbit pAb (A2435)

Western blot analysis of lysates from Mouse liver, using BRCA2 Rabbit pAb (A2435) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - BRCA2 Rabbit pAb (A2435)}

Immunohistochemistry - BRCA2 Rabbit pAb (A2435)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using BRCA2 Rabbit pAb (A2435) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - BRCA2 Rabbit pAb (A2435)}

Immunohistochemistry - BRCA2 Rabbit pAb (A2435)

Immunohistochemistry analysis of paraffin-embedded Human lung tissue using BRCA2 Rabbit pAb (A2435) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - BRCA2 Rabbit pAb (A2435)}

Immunofluorescence - BRCA2 Rabbit pAb (A2435)

Immunofluorescence analysis of C6 cells using BRCA2 Rabbit pAb (A2435) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2435? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)