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Bax Rabbit pAb (A12009)

Reviews (6)Publications (59) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Bax Rabbit pAb (A12009)

Western blot analysis of lysates from HeLa cells using Bax Rabbit pAb (A12009) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Bax Rabbit pAb (A12009)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells using Bax Rabbit pAb (A12009) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Bax Rabbit pAb (A12009)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit pAb (A12009) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of C6 cells using Bax Rabbit pAb (A12009) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of NIH/3T3 cells using Bax Rabbit pAb (A12009) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of NIH/3T3 cells using Bax Rabbit pAb (A12009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of PC-12 cells using Bax Rabbit pAb (A12009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of U2OS cells using Bax Rabbit pAb (A12009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Bax Rabbit pAb(A12009)
Review
ABclonal: review for Bax Rabbit pAb(A12009)
Review
ABclonal: review for Bax Rabbit pAb(A12009)
Review
ABclonal: review for Bax Rabbit pAb(A12009)
Review
ABclonal: review for Bax Rabbit pAb(A12009)
Review
ABclonal: review for Bax Rabbit pAb(A12009)

Overview

Product nameBax Rabbit pAb
Catalog No.A12009
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
BAX (also known as BCL2 Associated X, Bcl-2-Like Protein 4, Bcl2-L-4, BCL2L4) is a member of the BCL2 family of proteins that play a key role in the regulation of apoptosis in higher eukaryotes (https://www.uniprot.org/uniprot/Q07812). BAX comprises 4 Bcl-2 homology domains (BH1-BH4) and a C-terminal transmembrane domain. In healthy mammalian cells, BAX is localized to the cytoplasm through its interaction with the anti-apoptotic BL-2 family members BCL2L1/Bcl-xL . In response to apoptotic stimuli, however, BAX undergoes a conformational change that causes it to translocate to the outer mitochondrial membrane where it initiates the mitochondrial pathway of apoptosis via two potential mechanisms. Firstly, upon translocation to the outer mitochondrial membrane, BAX interacts with the mitochondrial voltage-dependent anion channel (VDAC) leading to the opening of the channel, loss of membrane potential, and the release of cytochrome c from the mitochondrion . The release of cytochrome C into the cytoplasm leads to the activation of Caspase3, initiating apoptosis. Secondly, activated BAX forms homodimers, which then assemble into oligomers on the mitochondrial outer membrane to create pores that permeabilize the mitochondrion leading to the release of cytochrome C.BAX has been shown to be involved in p53-mediated apoptosis. Expression of the human bax gene has been shown to be directly regulated by p53, and the bax promoter contains four motifs with homology to consensus p53-binding sites. Furthermore, p53 directly interacts with BAX to promote its activation.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
SequenceMDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMF
Gene ID
Swiss Prot
SynonymsBCL2 Associated X; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2L4; BAX
Calculated MW21kDa
Observed MW21kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T
Cellular locationCytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein.
Customer validation

(Human fibrosarcoma cell line, Mouse embryonal fibroblast cell, Human fibrosarcoma cells, rat cells, BEL-7402, HUVEC-P6)

WB(Rattus norvegicus, Mus musculus, Homo sapiens, Gallus gallus, Bos taurus, Cyprinus carpio, Mus musculus, Canis familiaris)

IHC(Mus musculus, Rattus norvegicus)

IHC(Homo sapiens)

IF(Homo sapiens, Bos taurus)

Co-IP(Mus musculus)

IF(Mus musculus, Homo sapiens)

FC(Bos taurus)

ELISA(Bos taurus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Bax Rabbit pAb (A12009)}

Western blot - Bax Rabbit pAb (A12009)

Western blot analysis of lysates from HeLa cells using Bax Rabbit pAb (A12009) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Bax Rabbit pAb (A12009)}

Western blot - Bax Rabbit pAb (A12009)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells using Bax Rabbit pAb (A12009) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Bax Rabbit pAb (A12009)}

Western blot - Bax Rabbit pAb (A12009)

Western blot analysis of lysates from wild type (WT) and Bax knockout (KO) 293T cells, using [KO Validated] Bax Rabbit pAb (A12009) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)}

Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of C6 cells using Bax Rabbit pAb (A12009) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)}

Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of NIH/3T3 cells using Bax Rabbit pAb (A12009) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)}

Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of NIH/3T3 cells using Bax Rabbit pAb (A12009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)}

Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of PC-12 cells using Bax Rabbit pAb (A12009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bax Rabbit pAb (A12009)}

Immunofluorescence - Bax Rabbit pAb (A12009)

Immunofluorescence analysis of U2OS cells using Bax Rabbit pAb (A12009) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (5)

ELISA 키트 (1)

Secondary Antibodies (26)