제품 > 항체 > 다중클론항체(pAb)

Bcl-2 Rabbit pAb (A0208)

Reviews (4)Publications (360) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Bcl-2 Rabbit pAb
ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of lysates from Mouse lung, using Bcl-2 Rabbit pAb (A0208) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of various lysates using Bcl-2 Rabbit pAb (A0208) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of various lysates using Bcl-2 Rabbit pAb (A0208) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of lysates from 293T cells, using Bcl-2 Rabbit pAb (A0208) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of lysates from Mouse lung using Bcl-2 Rabbit pAb (A0208) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)

Immunohistochemistry analysis of paraffin-embedded Human appendix using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of HeLa cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of U2OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of A-549 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)

Immunoprecipitation analysis of 200 μg extracts of THP-1 cells using 3 μg Bcl-2 antibody (A0208). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (A0208) at a dilution of 1:1000.

Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)
Review
ABclonal: review for Bcl-2 Rabbit pAb(A0208)

Overview

Product nameBcl-2 Rabbit pAb
Catalog No.A0208
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bcl-2 (NP_000624.2).
SequenceMAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQA
Gene ID
Swiss Prot
SynonymsBcl-2; PPP1R50
Calculated MW26kDa
Observed MW26kDa/
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, Mouse lung
Cellular locationEndoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein.
Customer validation

(rat cells, HCT116, B6, MCF-7,MD-MBA-231)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Gallus gallus, Bos taurus, Giardia duodenalis, Capra hircus, Ctenopharyngodon, Ctenopharyngodon idellus, quails, Sus scrofa, Cyprinus Carpio, Homo sapiens, Mus musculus, Eriocheir sinensis, Other, Rattus norvegicus, Oryctolagus cuniculus, Lampetra japonica)

IHC(Mus musculus, Homo sapiens, Oreochromis niloticus, Rattus norvegicus, Canis familiaris)

IF(Homo sapiens, Rattus norvegicus, Gallus gallus, Cyprinus carpio, Mus musculus)

IHC(Mus musculus, Homo sapiens, Gallus gallus)

IP(Mus musculus, Rattus norvegicus)

IF(Homo sapiens, Rattus norvegicus, Mus musculus)

IP(Homo sapiens, Mus musculus, Rattus norvegicus)

WB(Homo sapiens)

RT-PCR(Homo sapiens)

Other(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)}

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of lysates from Mouse lung, using Bcl-2 Rabbit pAb (A0208) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5s.
ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)}

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of various lysates using Bcl-2 Rabbit pAb (A0208) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)}

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of various lysates using Bcl-2 Rabbit pAb (A0208) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)}

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of lysates from 293T cells, using Bcl-2 Rabbit pAb (A0208) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - Bcl-2 Rabbit pAb (A0208)}

Western blot - Bcl-2 Rabbit pAb (A0208)

Western blot analysis of lysates from Mouse lung using Bcl-2 Rabbit pAb (A0208) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)}

Immunohistochemistry - Bcl-2 Rabbit pAb (A0208)

Immunohistochemistry analysis of paraffin-embedded Human appendix using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)}

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)}

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of HeLa cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)}

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)}

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of U2OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)}

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of A-549 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:250. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Bcl-2 Rabbit pAb (A0208)}

Immunofluorescence - Bcl-2 Rabbit pAb (A0208)

Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)}

Immunoprecipitation - Bcl-2 Rabbit pAb (A0208)

Immunoprecipitation analysis of 200 μg extracts of THP-1 cells using 3 μg Bcl-2 antibody (A0208). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (A0208) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (7)

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A19693

WBIHC-PIPELISA

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Bcl-2 Rabbit mAb

Bcl-2 Rabbit pAb
A11025

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

Bcl-2 Rabbit pAb

Bcl-2 Mouse mAb
A20777

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

Bcl-2 Mouse mAb

Phospho-Bcl-2-T56 Rabbit pAb
AP0316

ELISAWBIHC-P

Reactivity:Human, Mouse, Rat

Phospho-Bcl-2-T56 Rabbit pAb

Phospho-Bcl-2-S70 Rabbit pAb
AP0575

ELISAWBIF/ICC

Reactivity:Human, Rat

Phospho-Bcl-2-S70 Rabbit pAb

Phospho-Bcl-2-S87 Rabbit pAb
AP1071

ELISAWB

Reactivity:Human, Rat

Phospho-Bcl-2-S87 Rabbit pAb

[KD Validated] Bcl-2 Rabbit mAb
A21873

WBIHC-PIPELISA

Reactivity:Human

[KD Validated] Bcl-2 Rabbit mAb

ELISA 키트 (1)

Human B-cell leukemia/lymphoma 2 (BCL2) ELISA Kit
RK00971

Human B-cell leukemia/lymphoma 2 (BCL2) ELISA Kit

재조합 단백질 (2)

Recombinant Human Bcl-2 Protein
RP00062LQ

Tags:No tag

Expressed Host:E. coli

Recombinant Human Bcl-2 Protein

Recombinant Human Bcl-2 Protein
RP00062

Tags:No tag

Expressed Host:E. coli

Recombinant Human Bcl-2 Protein

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)