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Beclin 1 Rabbit pAb (A7353)

Reviews (6)Publications (113) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Beclin 1 Rabbit pAb
ABclonal:Western blot - Beclin 1 Rabbit pAb (A7353)

Western blot analysis of various lysates, using [KO Validated] Beclin 1 Rabbit pAb (A7353) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - Beclin 1 Rabbit pAb (A7353)

Western blot analysis of lysates from Mouse lung using [KO Validated] Beclin 1 Rabbit pAb (A7353) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Beclin 1 Rabbit pAb (A7353) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Beclin 1 Rabbit pAb (A7353) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Beclin 1 Rabbit pAb (A7353) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.

ABclonal:Immunoprecipitation - Beclin 1 Rabbit pAb (A7353)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg Beclin 1 antibody (A7353). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (A7353) at a dilution of 1:1000.

Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)
Review
ABclonal: review for Beclin 1 Rabbit pAb(A7353)

Overview

Product nameBeclin 1 Rabbit pAb
Catalog No.A7353
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
SequenceMEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSG
Gene ID
Swiss Prot
SynonymsATG6; VPS30; beclin1; Beclin 1
Calculated MW52kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:500
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samples293T, Mouse lung, Rat brain, HeLa
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, autophagosome, trans-Golgi network membrane.
Customer validation

(C2C12, A549,Mouse kidney, U2OS,Saos-2, Human Isikawa , NPCs)

WB(Mus musculus, Sus scrofa, Rattus norvegicus, Homo sapiens, Gallus gallus, Spodoptera frugiperda, Anatinae, Other, Horabagrus nigricollaris)

IF(Sus scrofa, Mus musculus, Rattus norvegicus)

IP(Nicotiana tabacum)

IHC(Homo sapiens,Rattus norvegicus, Mus musculus)

IF(Homo sapiens, Other, Mus musculus)

IHC(Mus musculus, Rattus norvegicus)

RT-PCR(Homo sapiens,Mus musculus)

WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Beclin 1 Rabbit pAb (A7353)}

Western blot - Beclin 1 Rabbit pAb (A7353)

Western blot analysis of various lysates, using [KO Validated] Beclin 1 Rabbit pAb (A7353) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - Beclin 1 Rabbit pAb (A7353)}

Western blot - Beclin 1 Rabbit pAb (A7353)

Western blot analysis of lysates from Mouse lung using [KO Validated] Beclin 1 Rabbit pAb (A7353) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)}

Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)

Immunohistochemistry analysis of paraffin-embedded Mouse intestin tissue using Beclin 1 Rabbit pAb (A7353) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)}

Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Beclin 1 Rabbit pAb (A7353) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)}

Immunohistochemistry - Beclin 1 Rabbit pAb (A7353)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Beclin 1 Rabbit pAb (A7353) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
ABclonal:Immunoprecipitation - Beclin 1 Rabbit pAb (A7353)}

Immunoprecipitation - Beclin 1 Rabbit pAb (A7353)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg Beclin 1 antibody (A7353). Western blot was performed from the immunoprecipitate using Beclin 1 antibody (A7353) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

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항체 (6)

Beclin 1 Rabbit pAb
A17028

ELISAWB

Reactivity:Human, Mouse, Rat

Beclin 1 Rabbit pAb

[KO Validated] Beclin 1 Rabbit mAb
A21191

ELISAWBIF/ICC

Reactivity:Human, Mouse, Rat

[KO Validated] Beclin 1 Rabbit mAb

Phospho-Beclin 1-Ser90 Rabbit pAb
AP1254

ELISAWB

Reactivity:Human, Mouse, Rat

Phospho-Beclin 1-Ser90 Rabbit pAb

[KO Validated] Beclin 1 Rabbit mAb
A22361

WBIF/ICCELISA

Reactivity:Human, Mouse, Rat

[KO Validated] Beclin 1 Rabbit mAb

Phospho-Beclin 1-Ser15 Rabbit pAb
AP1252

ELISAWB

Reactivity:Human, Mouse, Rat

Phospho-Beclin 1-Ser15 Rabbit pAb

Phospho-Beclin 1-Ser93 Rabbit pAb
AP1287

ELISAWB

Reactivity:Human

Phospho-Beclin 1-Ser93 Rabbit pAb

ELISA 키트 (1)

Human Beclin 1 ELISA Kit (BECN1)
RK00973

Human Beclin 1 ELISA Kit (BECN1)

Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)