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Brachyury Rabbit mAb (A5078)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Brachyury Rabbit mAb (A5078)

Western blot analysis of extracts of NCI-H460 cells, using Brachyury Rabbit mAb (A5078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Brachyury Rabbit mAb (A5078)

Western blot analysis of extracts of various cell lines, using Brachyury Rabbit mAb (A5078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - Brachyury Rabbit mAb (A5078)

Immunofluorescence analysis of NCI-H460 using Brachyury Rabbit mAb (A5078) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameBrachyury Rabbit mAb
Catalog No.A5078
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1983
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Variation in this gene was associated with susceptibility to neural tube defects and chordoma. A mutation in this gene was found in a family with sacral agenesis with vertebral anomalies.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 266-435 of human Brachyury (O15178).
SequenceGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM
Gene ID
Swiss Prot
SynonymsT; TFT; SAVA
Calculated MW47kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesNCI-H460, Mouse brain, Mouse thymus, Rat brain
Cellular locationNucleus

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ABclonal:Western blot - Brachyury Rabbit mAb (A5078)}

Western blot - Brachyury Rabbit mAb (A5078)

Western blot analysis of extracts of NCI-H460 cells, using Brachyury Rabbit mAb (A5078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Brachyury Rabbit mAb (A5078)}

Western blot - Brachyury Rabbit mAb (A5078)

Western blot analysis of extracts of various cell lines, using Brachyury Rabbit mAb (A5078) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunofluorescence - Brachyury Rabbit mAb (A5078)}

Immunofluorescence - Brachyury Rabbit mAb (A5078)

Immunofluorescence analysis of NCI-H460 using Brachyury Rabbit mAb (A5078) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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