This product is a universal SYBR Green reagent that uses a specially designed reference dye (ROX), has a more sensitive resolution, and can be applied to all types of fluorescence quantitative PCR instruments on the market (including High ROX, Low ROX, and No ROX required instruments). This reagent introduces a dUTP/UDG anti fouling system. UDG can quickly degrade pollutants containing U at room temperature, and UDG quickly deactivates when pre denatured at 95 ℃, without affecting the efficiency and sensitivity of qPCR. This product uses hot start Taq DNA polymerase for amplification, which greatly improves the specificity of the product while ensuring the amplification effect.
Product Features
Extremely sensitive: Adopting a brand new mixed enzyme formula, it has higher sensitivity, especially suitable for difficult samples and low abundance gene detection
Pollution prevention: With dUTP-UDG pollution prevention system, it can reduce the risk of aerosol pollution
Stability: The optimized buffer formula has better reagent stability, and there is no significant impact on performance when placed at 37 ℃ for one week. It can be transported in ice bags for a short time
High usability: Premixed with universal reference dyes (ROX), suitable for use on various instrument platforms without the need to add ROX separately
Applications
Dye-based qPCR
Storage
Store at -20℃. Protect from light
Product Information
Product Components
Components
catalog
Size1
Size 2
BrightCycle Universal SYBR Green qPCR Mix with UDG
RM21217
5 × 1 mL
25 × 1 mL
Product Data
1. 1.More advantageous in detecting difficult samples
The FFPE DNA sample and ChIP DNA sample are both complex samples with poor DNA integrity and low concentration. The FFPE DNA sample and ChIP DNA sample were qPCR detected using RK21219 and foreign competitors, respectively. The reaction procedures recommended in their respective manuals were used. The results showed that RK21219 had better sensitivity and amplification efficiency.
2. Stability testing for low abundance genes
When the Ct value is greater than 30, it is easy to have poor reproducibility of replicates, resulting in inaccurate detection results. Using RK21219 for qPCR detection of different types of low abundance samples, the results showed that the Ct values between different replicates are highly consistent, and the detection results are very stable.
3. Fine stability
Note: Template: Mouse liver RNA samples were reverse transcribed into cDNA, with RNA inputs of 100 ng, 10 ng, 1 ng, 100 pg, 10 pg, H2O, and the gene mGAPDH was detected. The black curve is the control, and the red curve is the test to be tested
Publishing research using RK21219? Please let us know so that we can cite the reference in this datasheet.
Beneficial impact of cardiac heavy metal scavenger metallothionein in sepsis-provoked cardiac anomalies dependent upon regulation of endoplasmic reticulum stress
Identification of diagnostic markers related to inflammatory response and cellular senescence in endometriosis using machine learning and in vitro experiment
Published:
2024
Journal:
Inflammation research : official journal of the European Histamine Research Society ... [et al.]
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