Review (1)Publications (31) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from A-549 cells, using CCL2/MCP-1 Rabbit pAb (A7277) at 1:2000 dilution. A-549 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
Western blot analysis of Recombinant Human CCL2/MCP-1 Protein (RP01653), using CCL2/MCP-1 Rabbit pAb (A7277) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 10ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using CCL2/MCP-1 Rabbit pAb (A7277) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CCL2/MCP-1 Rabbit pAb (A7277) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CCL2/MCP-1 Rabbit pAb (A7277) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
| Product name | CCL2/MCP-1 Rabbit pAb |
|---|---|
| Catalog No. | A7277 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 24-99 of human CCL2/MCP-1 (NP_002973.1). |
|---|---|
| Sequence | QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30; CCL2/MCP-1 |
| Calculated MW | 11kDa |
| Observed MW | 13-15kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | A-549 treated by TNF-α, Recombinant Human CCL2/MCP-1 Protein |
| Cellular location | Secreted. |
| Customer validation | (rat brain cells) WB(Mus musculus, Homo sapiens, Rattus norvegicus, Mus musculus, Ovis aries) IHC(Mus musculus, Mus musculus , Homo sapiens, Mus musculus) IHC(Homo sapiens, Rattus norvegicus, Mus musculus) IF(Homo sapiens, Mus musculus) |
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_WB_01.jpg?t=1721722881 "ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)")
_WB_02.jpg?t=1721722881 "ABclonal:Western blot - CCL2/MCP-1 Rabbit pAb (A7277)")
