제품 > 항체 > 다중클론항체(pAb)

CD117/c-Kit Rabbit pAb (A0357)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse lung, using CD117/c-Kit Rabbit pAb (A0357) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using CD117/c-Kit Rabbit pAb (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse intestin using CD117/c-Kit Rabbit pAb (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD117/c-Kit Rabbit pAb (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat kidney using CD117/c-Kit Rabbit pAb (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat intestine using CD117/c-Kit Rabbit pAb (A0357) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)

Immunofluorescence analysis of NIH/3T3 cells using CD117/c-Kit Rabbit pAb (A0357) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using CD117/c-Kit Rabbit pAb (A0357) at dilution of 1:50. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	CD117/c-Kit Rabbit pAb
Catalog No.	A0357
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 210-412 of human CD117/c-Kit (NP_000213.1).
Sequence	AVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTK
Gene ID
Swiss Prot
Synonyms	PBT; SCFR; C-Kit; CD117; MASTC; CD117/c-Kit
Calculated MW	110kDa
Observed MW	145kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse lung
Cellular location	Cell membrane, Cytoplasm, Single-pass type I membrane protein
Customer validation	WB(Homo sapiens, Mus musculus) IHC(Rattus norvegicus) IF(Mus musculus, Homo sapiens) mIHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

CD117/c-Kit Rabbit pAb images

Western blot - CD117/c-Kit Rabbit pAb (A0357)

Immunohistochemistry - CD117/c-Kit Rabbit pAb (A0357)

Immunofluorescence - CD117/c-Kit Rabbit pAb (A0357)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

Biological Research

Application:

IF:

4.3

Species:

Homo sapiens

PMID:

32209121

Title:

Mast cells-derived MiR-223 destroys intestinal barrier function by inhibition of CLDN8 expression in intestinal epithelial cells

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

Communications biology

Application:

IHC

IF:

6.5

Species:

Rattus norvegicus

PMID:

36085351

Title:

Integrated multi-omics reveal important roles of gut contents in intestinal ischemia-reperfusion induced injuries in rats - PMC

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

Journal of personalized medicine

Application:

IF:

3.5

Species:

Mus musculus

PMID:

36675807

Title:

Microglia-Derived Spp1 Promotes Pathological Retinal Neovascularization via Activating Endothelial Kit/Akt/mTOR Signaling - PMC

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Mus musculus

PMID:

37063880

Title:

The macrophages regulate intestinal motility dysfunction through the PGE2 Ptger3 axis during Klebsiella pneumonia sepsis - PMC

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

Nucleic acids research

Application:

IF:

16.6

Species:

Homo sapiens

PMID:

37739418

Title:

The arginine methyltransferase Prmt1 coordinates the germline arginine methylome essential for spermatogonial homeostasis and male fertility

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

38169659

Title:

New insights from integrated bioinformatics analysis: the role of circadian rhythm disruption and immune infiltration in obstructive sleep apnea disease - PMC

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

Microscopy research and technique

Application:

IHC

IF:

2.5

Species:

Rattus norvegicus

PMID:

38441397

Title:

Role of mesenchymal stem cellsderived exosomes on inflammation, apoptosis, fibrosis and telocyte modulation in doxorubicininduced cardiotoxicity: A closer look

References for CD117/c-Kit Rabbit pAb (A0357)

Product:

CD117/c-Kit Rabbit pAb

Journal:

The EMBO journal

Application:

IF:

9.4

Species:

Homo sapiens

PMID:

38750259