제품 > 항체 > 단일클론항체(mAb)

CD130/gp130 Rabbit mAb (A24991)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 Neuro-2a cells (Low Expression, left) and NIH/3T3 cells (right) were surface-stained with CD130/gp130 Rabbit mAb (A24991, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 NIH/3T3 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD130/gp130 Rabbit mAb (A24991, 2 μg/mL, right).

Overview

Product name	CD130/gp130 Rabbit mAb
Catalog No.	A24991
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC64569

Background

Contributes to interleukin-11 binding activity and interleukin-11 receptor activity. Involved in interleukin-6-mediated signaling pathway; intestinal epithelial cell development; and positive regulation of Notch signaling pathway. Acts upstream of or within several processes, including glycogen metabolic process; positive regulation of astrocyte differentiation; and regulation of Notch signaling pathway. Located in dendrite; external side of plasma membrane; and neuronal cell body. Is expressed in several structures, including alimentary system; brain; early conceptus; immune system; and reproductive system. Used to study rheumatoid arthritis and stomach cancer. Human ortholog(s) of this gene implicated in arteriosclerosis; hyper IgE recurrent infection syndrome 4; and hyperandrogenism. Orthologous to human IL6ST (interleukin 6 cytokine family signal transducer).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 23-617 of mouse CD130/gp130 (NP_034690.3).
Sequence	QLLEPCGYIYPEFPVVQRGSNFTAICVLKEACLQHYYVNASYIVWKTNHAAVPREQVTVINRTTSSVTFTDVVLPSVQLTCNILSFGQIEQNVYGVTMLSGFPPDKPTNLTCIVNEGKNMLCQWDPGRETYLETNYTLKSEWATEKFPDCQSKHGTSCMVSYMPTYYVNIEVWVEAENALGKVSSESINFDPVDKVKPTPPYNLSVTNSEELSSILKLSWVSSGLGGLLDLKSDIQYRTKDASTWIQVPLEDTMSPRTSFTVQDLKPFTEYVFRIRSIKDSGKGYWSDWSEEASGTTYEDRPSRPPSFWYKTNPSHGQEYRSVRLIWKALPLSEANGKILDYEVILTQSKSVSQTYTVTGTELTVNLTNDRYVASLAARNKVGKSAAAVLTIPSPHVTAAYSVVNLKAFPKDNLLWVEWTPPPKPVSKYILEWCVLSENAPCVEDWQQEDATVNRTHLRGRLLESKCYQITVTPVFATGPGGSESLKAYLKQAAPARGPTVRTKKVGKNEAVLAWDQIPVDDQNGFIRNYSISYRTSVGKEMVVHVDSSHTEYTLSSLSSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEIE
Gene ID
Swiss Prot
Synonyms	CD130; gp130; D13Ertd699e; 5133400A03Rik; CD130/gp130
Calculated MW	102kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

CD130/gp130 Rabbit mAb images

Flow CytoMetry - CD130/gp130 Rabbit mAb (A24991)

Flow cytometry: 1X10^6 NIH/3T3 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD130/gp130 Rabbit mAb (A24991, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.