제품 > 항체 > 다중클론항체(pAb)

CD31/PECAM1 Rabbit pAb (A0378)

Publications (56) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of lysates from THP-1 cells, using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of lysates from Mouse heart, using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of various lysates using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of lysates from U-937 cells, using CD31/PECAM1 Rabbit pAb (A0378) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A0378)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD31/PECAM1 Rabbit pAb (A0378) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A0378)

Immunohistochemistry analysis of paraffin-embedded Human placenta using CD31/PECAM1 Rabbit pAb (A0378) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD31/PECAM1 Rabbit pAb (A0378)

Immunofluorescence analysis of Human placenta tissue using CD31/PECAM1 Rabbit pAb (A0378) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

Overview

Product nameCD31/PECAM1 Rabbit pAb
Catalog No.A0378
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 630-738 of CD31/PECAM (NP_000433.4).
SequenceAKQMPVEMSRPAVPLLNSNNEKMSDPNMEANSHYGHNDDVRNHAMKPINDNKEPLNSDVQYTEVQVSSAESHKDLGKKDTETVYSEVRKAVPDAVESRYSRTEGSLDGT
Gene ID
Swiss Prot
SynonymsCD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Calculated MW83kDa
Observed MW140kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, Mouse heart
Cellular locationCell junction, Cell junction, Cell membrane, Lipid-anchor, Single-pass type I membrane protein.
Customer validation

IF(Rattus norvegicus, Mus musculus, Macaca fascicularis)

IHC(Rattus norvegicus, Homo sapiens, Mus musculus, Mus musculus)

WB(Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus, Homo sapiens, Gallus gallus)

IF(Rattus norvegicus, Sus scrofa, Mus musculus, Gallus gallus, Homo sapiens)

IHC(Homo sapiens, Rattus norvegicus)

FC(Homo sapiens)

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)}

Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of lysates from THP-1 cells, using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)}

Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of lysates from Mouse heart, using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)}

Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of various lysates using CD31/PECAM1 Rabbit pAb (A0378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - CD31/PECAM1 Rabbit pAb (A0378)}

Western blot - CD31/PECAM1 Rabbit pAb (A0378)

Western blot analysis of lysates from U-937 cells, using CD31/PECAM1 Rabbit pAb (A0378) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A0378)}

Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A0378)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD31/PECAM1 Rabbit pAb (A0378) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A0378)}

Immunohistochemistry - CD31/PECAM1 Rabbit pAb (A0378)

Immunohistochemistry analysis of paraffin-embedded Human placenta using CD31/PECAM1 Rabbit pAb (A0378) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CD31/PECAM1 Rabbit pAb (A0378)}

Immunofluorescence - CD31/PECAM1 Rabbit pAb (A0378)

Immunofluorescence analysis of Human placenta tissue using CD31/PECAM1 Rabbit pAb (A0378) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0378? Please let us know so that we can cite the reference in this datasheet.

항체 (8)

CD31/PECAM1 Rabbit mAb
A19014

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse, Rat

CD31/PECAM1 Rabbit mAb

CD31/PECAM1 Rabbit pAb
A2104

WBIF/ICCELISA

Reactivity:Human, Mouse, Rat

CD31/PECAM1 Rabbit pAb

CD31/PECAM1 Rabbit mAb
A18643

WBIF/ICCIPELISA

Reactivity:Human

CD31/PECAM1 Rabbit mAb

CD31/PECAM1 Rabbit mAb
A20228

WBIHC-PIPELISA

Reactivity:Human

CD31/PECAM1 Rabbit mAb

ABflo® 488 Rabbit anti-Human CD31/PECAM1 mAb
A22508

FC

Reactivity:Human

ABflo® 488 Rabbit anti-Human CD31/PECAM1 mAb

ABflo® 647 Rabbit anti-Human CD31/PECAM1 mAb
A22509

FC

Reactivity:Human

ABflo® 647 Rabbit anti-Human CD31/PECAM1 mAb

ABflo® 700 Rabbit anti-Human CD31/PECAM1 mAb
A26238

FC

Reactivity:Human

ABflo® 700 Rabbit anti-Human CD31/PECAM1 mAb

CD31/PECAM1 Mouse mAb
A27114

WBIHC-PIF/ICCELISA

Reactivity:Human, Mouse

CD31/PECAM1 Mouse mAb

재조합 단백질 (1)

Recombinant Human PECAM-1/CD31 Protein
RP00096

Tags:C-His

Expressed Host:HEK293 cells

Recombinant Human PECAM-1/CD31 Protein

Secondary Antibodies (25)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb