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CD45 Rabbit mAb (A23503)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

The multiplex IHC analysis on paraffin-embedded Human breast cancer tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD45 Rabbit mAb (A23503, 1:5000) with TSA-TYR-520 (Green), CD8A Rabbit mAb (A23305PM, 1:1000) with TSA-TYR-570 (Red), and CD3E Rabbit mAb (A24060, 1:200) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

The multiplex IHC analysis on paraffin-embedded Human colon tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD45 Rabbit mAb (A23503, 1:5000) with TSA-TYR-520 (Green), CD8A Rabbit mAb (A23305PM, 1:1000) with TSA-TYR-570 (Red), and CD3E Rabbit mAb (A24060, 1:200) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

The multiplex IHC analysis on paraffin-embedded Human tonsil tissue using the following specific primary antibodies and tyramide signal amplification (TSA) reagents (RK05903) : CD45 Rabbit mAb (A23503, 1:5000) with TSA-TYR-520 (Green), CD8A Rabbit mAb (A23305PM, 1:1000) with TSA-TYR-570 (Red), and CD3E Rabbit mAb (A24060, 1:200) with TSA-TYR-690 (Magenta). DAPI (Blue) was used for nuclear staining. Prior to multiplex IHC staining, high-pressure antigen retrieval was performed using 0.01M citrate buffer at pH 6.0. The analysis was completed using a 20x objective lens.

ABclonal:Western blot - CD45 Rabbit mAb (A23503)

Western blot analysis of various lysates, using CD45 Rabbit mAb (A23503) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):MCF7
Exposure time: 1s.

ABclonal:Immunohistochemistry - CD45 Rabbit mAb (A23503)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CD45 Rabbit mAb (A23503) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using CD45 Rabbit mAb (A23503) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using CD45 Rabbit mAb (A23503) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD45 Rabbit mAb (A23503) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using CD45 Rabbit mAb (A23503) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product name	CD45 Rabbit mAb
Catalog No.	A23503
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC52280

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Immunogen information

Immunogen	Recombinant Protein corresponding to a sequence within amino acids 194-390 of human CD45 (NP_002829.3).
Sequence	SDAYLNASETTTLSPSGSAVISTTTIATTPSKPTCDEKYANITVDYLYNKETKLFTAKLNVNENVECGNNTCTNNEVHNLTECKNASVSISHNSCTAPDKTLILDVPPGVEKFQLHDCTQVEKADTTICLKWKNIETFTCDTQNITYRFQCGNMIFDNKEIKLENLEPEHEYKCDSEILYNNHKFTNASKIIKTDFG
Gene ID
Swiss Prot
Synonyms	LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; IMD105
Calculated MW	147kDa
Observed MW	180-240kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100-1:500 IHC-P 1:5000 - 1:20000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Jurkat
Cellular location	Membrane, Membrane raft, Single-pass type I membrane protein.